| Texto completo | |
| Autor(es): |
Pimentaa, Leticia Pereira
;
Kellner Filho, Luis Cladio
;
Soares Parpinelli, Bruna Arruda
;
de Siqueira, Katia Aparecida
;
Soares, Marcos Antonio
;
Andrade e Silva, Marcio Luis
;
Cunha, Wilson Roberto
;
Pauletti, Patricia Mendonca
;
Januario, Ana Helena
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Química Nova; v. 40, n. 9, p. 5-pg., 2017-09-01. |
| Resumo | |
Vochysia divergens Pohl, known as "Cambara" in Brazil, is an invasive species that is expanding throughout Pantanal in Brazil, to form mono-dominant communities. This expansion is affecting the agricultural areas that support the typical seasonal flood and drought conditions of this biome. This article describes the development and validation of an HPLC-DAD analytical method to quantify 5-methoxyflavones in methanolic extracts of greenhouse-grown V. divergens associated with one of two endophytic fungal species Zopfiella tetraspora (Zt) or Melanconiella elegans (Me) and later subjected to water stress. The developed method gave good validation parameters and was successfully applied to quantify the flavones 3', 5-dimethoxy luteolin-7-O-beta-glucopyranoside (1), 5-methoxy luteolin (2), and 3', 5-dimethoxy luteolin (3) in the target extracts. Inoculation of the plant with Zt decreased the concentration of flavone 1 in the extract by 2.69-fold as compared to the control. Inoculation of the plant with Zt or Me did not significantly alter the contents of flavones 2 and 3 in the extracts as compared to the control. Therefore, the aerial parts of germinated V. divergens plants inoculated with either Zt or Me responded differently in terms of the production of flavones. These results can cast light on the symbiosis between fungal microorganisms and V. divergens, which most likely influences the response of V. divergens to changes in the availability of water in Pantanal. (AU) | |
| Processo FAPESP: | 13/22030-9 - Avaliação do perfil químico por HPLC/DAD/MS de plantas de Vochysia divergens cultivadas sob estresse hídrico associadas com fungos endofíticos e avaliação das atividades antioxidante e neuroprotetora |
| Beneficiário: | Letícia Pereira Pimenta |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 11/00631-5 - Compostos bioativos de plantas medicinais e alimentícias do cerrado e Pantanal e de sua microbiota endofítica |
| Beneficiário: | Ana Helena Januário |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 16/08057-0 - Estudo químico do caule Vochysia divergens por HPLC/DAD/MS e avaliação das atividades esquistossomicida, acetilcolinasterásica e antioxidante |
| Beneficiário: | Letícia Pereira Pimenta |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 13/15774-1 - Estudo químico da fração AcOEt de Vochysia divergens e avaliação da ação esquistossomicida in vitro |
| Beneficiário: | Luis Claudio Kellner Filho |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |