Neurospora crassa developmental control mediated by the FLB-3 transcription factor

Autor(es):Mostrar menos -

Boni, Ana Carolina ; Ambrosio, Daniela Luz ; Cupertino, Fernanda Barbosa ; Montenegro-Montero, Alejandro ; Virgilio, Stela ; Freitas, Fernanda Zanolli ; Corrocher, Flavia Adolfo ; Goncalves, Rodrigo Duarte ; Yang, Ally ; Weirauch, Matthew T. ; Hughes, Timothy R. ; Larrondo, Luis F. ; Bertolini, Maria Celia

• Tipo de documento: Artigo Científico
• Fonte: FUNGAL BIOLOGY; v. 122, n. 6, p. 13-pg., 2018-06-01.

Resumo

Here, we report that the Neurospora crassa FLB-3 protein, the ortholog of the Aspergillus nidulans FIbC transcription factor, is required for developmental control. Deletion of flb-3 leads to changes in hyphae morphology and affects sexual and asexual development. We identified, as putative FLB-3 targets, the N. crassa aba-1, wet-1 and vos-1 genes, orthologs of the ones involved in A. nidulans asexual development and that work downstream of FIbC (abaA, wetA and vosA). In N. crassa, these three genes require FLB-3 for proper expression; however, they appear not to be required for normal development, as demonstrated by gene expression analyses during vegetative growth and asexual development. Moreover, mutant strains in the three genes conidiate well and produce viable conidia. We also determined FLB-3 DNA-binding preferences via protein-binding microarrays (PBMs) and demonstrated by chromatin immunoprecipitation (ChIP) that FLB-3 binds the aba-1, wet-1 and vos-1 promoters. Our data support an important role for FLB-3 in N. crassa development and highlight differences between the regulatory pathways controlled by this transcription factor in different fungal species. (C) 2018 British Mycological Society. Published by Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 13/24705-3 - O fungo filamentoso Neurospora crassa como um organismo modelo para a caracterização funcional de proteínas/fatores de transcrição que regulam o metabolismo de carboidratos
• Beneficiário: Maria Celia Bertolini
• Modalidade de apoio: Auxílio à Pesquisa - Temático