Low root PIP1-1 and PIP2 aquaporins expression could be related to reduced hydration in 'Rangpur' lime plants exposed to aluminium

Autor(es)

Cavalheiro, Mariana F. ; Gavassi, Marina A. ; Silva, Giselle S. ; Nogueira, Matheus A. ; Silva, Carolina M. S. ; Domingues, Douglas S. ; Habermann, Gustavo

• Tipo de documento: Artigo Científico
• Fonte: FUNCTIONAL PLANT BIOLOGY; v. 47, n. 2, p. 10-pg., 2020-01-01.

Resumo

In acidic soils, aluminium (Al) occurs as Al3+, which is phytotoxic. One of the most conspicuous symptoms of Al toxicity is the root growth inhibition, which can lead to low water uptake and consequent reduction in leaf hydration and gas exchange. However, fibrous xylem vessels have been observed in roots of 'Rangpur' lime plants (Citrus limonia L.) when exposed to Al, which could affect the functioning of aquaporins, ultimately reducing their expression. We confirmed a decrease of CO2 assimilation (A), stomatal conductance (g(s)), transpiration (E) and relative leaf water content (RWC) in 3-month-old C. limonia plants exposed to 1480 mu M Al in nutrient solution for 90 days. The estimated hydraulic conductivity from soil to the leaf (K-L) and leaf water potential (psi w) also showed low values, although not consistently reduced over time of Al exposure. The relative expression of aquaporin genes belonging to PIP family (PIP1-1, PIP1-2 and PIP2) showed downregulation for ClPIP1-1 and ClPIP2 and upregulation for ClPIP1-2 in plants exposed to Al. Furthermore, ClPIP1-1 was positively correlated with A and g(s) in plants exposed to Al. Therefore, downregulation of ClPIP1-1 and ClPIP2 in roots of 'Rangpur' lime plants could be associated with the low leaf hydration of this species when exposed to Al. (AU)

• Processo FAPESP: 16/10896-0 - A sinalização do ácido hexanóico pode modular o transcriptoma, metaboloma e o desenvolvimento de patógenos no cafeeiro?
• Beneficiário: Douglas Silva Domingues
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 13/11370-3 - Expressão gênica em mecanismos de tolerância ao Al3+ comparando espécie do Cerrado (Qualea grandiflora) e limoeiro (Citrus limonia cv. 'Cravo')
• Beneficiário: Carolina de Marchi Santiago da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/26144-0 - Aspectos hidráulicos, metabólicos e moleculares da interferência do Al3+ na hidratação da parte aérea em Citrus limonia
• Beneficiário: Gustavo Habermann
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/25409-4 - Papel do ABA nas relações hídricas em metabolismos contrastantes à sensibilidade ao Al3+
• Beneficiário: Marina Alves Gavassi
• Modalidade de apoio: Bolsas no Brasil - Doutorado