Evaluation of Elafin Immunohistochemical Expression as Marker of Cervical Cancer Severity

Autor(es)

Longatto-Filho, Adhemar ; Fregnani, Jose Humberto ; da Costa, Allini Mafra ; de Araujo-Souza, Patricia Savio ; Scapulatempo-Neto, Cristovam ; Herbster, Suellen ; Boccardo, Enrique ; Termini, Lara

• Tipo de documento: Artigo Científico
• Fonte: ACTA CYTOLOGICA; v. 65, n. 2, p. 10-pg., 2020-12-03.

Resumo

Introduction: The main risk factor for the development of cervical cancer (CC) is persistent infection by human papillomavirus (HPV) oncogenic types. In order to persist, HPV exhibits a plethora of immune evasion mechanisms. PI3/Elafin (Peptidase Inhibitor 3) is an endogenous serine protease inhibitor involved in epithelial protection against pathogens. PI3/Elafin's role in CC is still poorly understood. Materials and Methods: In the present study, we addressed PI3/Elafin protein detection in 123 CC samples by immunohistochemistry and mRNA expression in several datasets available at Gene Expression Omnibus and The Cancer Genome Atlas platforms. Results: We observed that PI3/Elafin is consistently downregulated in CC samples when compared to normal tissue. Most of PI3/Elafin-positive samples exhibited this protein at the plasma membrane. Besides, high PI3/Elafin expression at the cellular membrane was more frequent in in situ stages I + II than in invasive cervical tumor stages III + IV. This indicates that PI3/Elafin expression is gradually lost during the CC progression. Of note, advanced stages of CC were more frequently associated with a more intense PI3/Elafin reaction in the nuclei and cytoplasm. Conclusion: Our results suggest that PI3/Elafin levels and subcellular localization may be used as a biomarker for CC severity. (AU)

• Processo FAPESP: 08/57889-1 - Instituto de Ciência e Tecnologia para o Estudo das Doenças Associadas ao Papilomavírus
• Beneficiário: Luisa Lina Villa
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/22097-0 - Neoplasias malignas nas 18 cidades pertencentes à regional de saúde de Barretos, São Paulo: a importância de um Registro de Câncer de Base Populacional
• Beneficiário: Allini Mafra da Costa
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 05/57274-9 - Identificação de marcadores de progressão tumoral em amostras do colo uterino e sua associação com a infecção por diferentes tipos de HPV
• Beneficiário: Lara Termini
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado