| Texto completo | |
| Autor(es): |
Longatto-Filho, Adhemar
;
Fregnani, Jose Humberto
;
da Costa, Allini Mafra
;
de Araujo-Souza, Patricia Savio
;
Scapulatempo-Neto, Cristovam
;
Herbster, Suellen
;
Boccardo, Enrique
;
Termini, Lara
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ACTA CYTOLOGICA; v. 65, n. 2, p. 10-pg., 2020-12-03. |
| Resumo | |
Introduction: The main risk factor for the development of cervical cancer (CC) is persistent infection by human papillomavirus (HPV) oncogenic types. In order to persist, HPV exhibits a plethora of immune evasion mechanisms. PI3/Elafin (Peptidase Inhibitor 3) is an endogenous serine protease inhibitor involved in epithelial protection against pathogens. PI3/Elafin's role in CC is still poorly understood. Materials and Methods: In the present study, we addressed PI3/Elafin protein detection in 123 CC samples by immunohistochemistry and mRNA expression in several datasets available at Gene Expression Omnibus and The Cancer Genome Atlas platforms. Results: We observed that PI3/Elafin is consistently downregulated in CC samples when compared to normal tissue. Most of PI3/Elafin-positive samples exhibited this protein at the plasma membrane. Besides, high PI3/Elafin expression at the cellular membrane was more frequent in in situ stages I + II than in invasive cervical tumor stages III + IV. This indicates that PI3/Elafin expression is gradually lost during the CC progression. Of note, advanced stages of CC were more frequently associated with a more intense PI3/Elafin reaction in the nuclei and cytoplasm. Conclusion: Our results suggest that PI3/Elafin levels and subcellular localization may be used as a biomarker for CC severity. (AU) | |
| Processo FAPESP: | 08/57889-1 - Instituto de Ciência e Tecnologia para o Estudo das Doenças Associadas ao Papilomavírus |
| Beneficiário: | Luisa Lina Villa |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 18/22097-0 - Neoplasias malignas nas 18 cidades pertencentes à regional de saúde de Barretos, São Paulo: a importância de um Registro de Câncer de Base Populacional |
| Beneficiário: | Allini Mafra da Costa |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 05/57274-9 - Identificação de marcadores de progressão tumoral em amostras do colo uterino e sua associação com a infecção por diferentes tipos de HPV |
| Beneficiário: | Lara Termini |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |