Light excess stimulates Poly-beta-hydroxybutyrate yield in a mangrove-isolated strain of Synechocystis sp.

Autor(es)

Gracioso, Louise Hase ; Bellan, Alessandra ; Karolski, Bruno ; Bispo Cardoso, Leticia Oliveira ; Perpetuo, Elen Aquino ; Oller do Nascimento, Claudio Augusto ; Giudici, Reinaldo ; Pizzocchero, Valentino ; Basaglia, Marina ; Morosinotto, Tomas

• Tipo de documento: Artigo Científico
• Fonte: Bioresource Technology; v. 320, p. 7-pg., 2021-01-01.

Resumo

Poly-beta-hydroxybutyrate (PHB) is a biodegradable biopolymer that may replace fossil-based plastics reducing its negative environmental impact. One highly sustainable strategy to produce these biopolymers is the exploitation of photosynthetic microorganisms that use sunlight and CO2 to produce biomass and subsequently, PHB. Exploring environmental biological diversity is a powerful tool to find resilient microorganisms potentially exploitable to produce bioproducts. In this work, a cyanobacterium (Synechocystis sp.) isolated from a contaminated area close to an important industrial complex was shown to produce PHB under different culture conditions. Carbon, nutrients supply and light intensity impact on biomass and PHB productivity were assessed, showing that the highest yield of PHB achieved was 241 mg L-1 (31%dcw) under high light intensity. Remarkably this condition not only stimulated PHB accumulation by 70% compared to other conditions tested but also high cellular duplication rate, maximizing the potential of this strain for PHB production. (AU)

• Processo FAPESP: 17/17438-0 - Mitigação de CO2 e CH4 através do uso de consórcio microbiano (algas e bactérias)
• Beneficiário: Louise Hase Gracioso
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/10811-0 - Otimização de cultivo de microalgas para indução de produção de moléculas de alto valor
• Beneficiário: Louise Hase Gracioso
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 14/50279-4 - Brasil Research Centre for Gas Innovation
• Beneficiário: Julio Romano Meneghini
• Modalidade de apoio: Auxílio à Pesquisa - Programa Centros de Pesquisa em Engenharia
• Processo FAPESP: 18/22790-7 - Avaliação do potencial da produção de biopolímeros por bactérias metilotróficas
• Beneficiário: Elen Aquino Perpetuo
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular