Busca avançada
Ano de início
Entree
Conteúdo relacionado
(Referência obtida automaticamente do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

RAPID METHOD FOR SIMULTANEOUS DETERMINATION OF CIS-9, TRANS-11 AND TRANS-10, CIS-12 CONJUGATED LINOLEIC ACID ISOMERS IN MILK BY GC-FID

Texto completo
Autor(es):
Brenda Lee Simas Porto ; Isaura Daniele Leite Faria [2] ; Jéssica Cordeiro Queiroz de Souza [3] ; Olívia Brito de Oliveira Moreira [4] ; Marco Antônio Sundfeld da Gama [5] ; Marcone Augusto Leal de Oliveira
Número total de Autores: 6
Tipo de documento: Artigo Científico
Fonte: Química Nova; v. 46, n. 7, p. 743-746, 2023-08-28.
Resumo

Ruminant milk is the lead source of conjugated linoleic acid (CLA) in the human diet, with cis-9, trans-11 CLA being the major among all. Small amounts of trans-10, cis-12 CLA are also found in synthetic supplements. Since both isomers are biologically active with potential health benefits, there is great interest in quantifying them for quality control routines. An alternative method for the analysis of the aforementioned CLAs by fast gas chromatography (GC) is discussed in the present study. The fatty acid methyl ester mixture obtained by alkaline catalysis was injected into a GC equipped with a fame ionization detector (FID) and fitted with an ionic liquid SLB-IL111 chromatographic column (15 m × 0.10 mm × 0.08 µm). Separation was achieved in less than 5 min using a 168 °C isotherm run. Both CLA isomers were quantified by using of single point standard addition statistical approach. Results were contested to those obtained using the recommended 100 m long CP-Sil88 capillary column and none evidence of significant differences was found within 95% confidence interval. Therefore, the proposed method could be valuable to focused regulatory routines of large numbers of samples with greater analytical frequency. (AU)

Processo FAPESP: 14/50867-3 - INCT 2014: Instituto Nacional de Ciência e Tecnologia de Bioanalítica
Beneficiário:Marco Aurelio Zezzi Arruda
Modalidade de apoio: Auxílio à Pesquisa - Temático