Differential Impact of Adolescent or Adult Stress on Behavior and Cortical Parvalbumin Interneurons and Perineuronal Nets in Male and Female Mice

Autor(es)

Santos-Silva, Thamyris ; Souza, Beatriz Kinchin ; Colodete, Debora Akemi Endo ; Campos, Lara Ramos ; Lima, Thais Santos Almeida ; Guimaraes, Francisco S. ; Gomes, Felipe, V

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF NEUROPSYCHOPHARMACOLOGY; v. 27, n. 10, p. 13-pg., 2024-10-18.

Resumo

Background Stress has become a common public health concern, contributing to the rising prevalence of psychiatric disorders. Understanding the impact of stress considering critical variables, such as age, sex, and individual differences, is of the utmost importance for developing effective intervention strategies.Methods Stress effects (daily footshocks for 10 days) during adolescence (postnatal day [PND] 31-40) and adulthood (PND 65-74) were investigated on behavioral outcomes and parvalbumin (PV)-expressing GABAergic interneurons and their associated perineuronal nets (PNNs) in the prefrontal cortex of male and female mice 5 weeks post stress.Results In adulthood, adolescent stress induced behavioral alterations in male mice, including anxiety-like behaviors, social deficits, cognitive impairments, and altered dopamine system responsivity. Applying integrated behavioral z-score analysis, we identified sex-specific differences in response to adolescent stress, with males displaying greater vulnerability than females. Furthermore, adolescent-stressed male mice showed decreased PV+ and PNN+ cell numbers and PV+/PNN+ colocalization, while in females, adolescent stress reduced prefrontal PV+/PNN+ colocalization in the prefrontal cortex. Further analysis identified distinct behavioral clusters, with certain females demonstrating resilience to adolescent stress-induced deficits in sociability and PV+ cell number. Adult stress in male and female mice did not cause long-lasting changes in behavior and PV+ and PNN+ cell number.Conclusion Our findings indicate that the timing of stress, sex, and individual variabilities seem to be determinants for the development of behavioral changes associated with psychiatric disorders, particularly in male mice during adolescence. (AU)

• Processo FAPESP: 22/10753-5 - Avaliação de alterações comportamentais induzidas pela exposição ao estresse durante os períodos pré/peripubertal ou adulto.
• Beneficiário: Beatriz Kinchin de Souza
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 18/17597-3 - O impacto do estresse sobre o sistema dopaminérgico é determinado pelo período crítico de plasticidade: implicações para a depressão e a esquizofrenia e o estudo de novos alvos farmacológicos
• Beneficiário: Felipe Villela Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 17/24304-0 - Novas perspectivas no emprego de fármacos que modificam neurotransmissores atípicos no tratamento de transtornos neuropsiquiátricos
• Beneficiário: Francisco Silveira Guimaraes
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 21/03391-7 - Estudo da reabertura do período sensível de suscetibilidade ao estresse pela degradação das redes perineuronais
• Beneficiário: Debora Akemi Endo Colodete
• Modalidade de apoio: Bolsas no Brasil - Mestrado