| Texto completo | |
| Autor(es): |
Souza, Alann Thaffarell Portilho
;
Freitas, Gileade Pereira
;
Lopes, Helena Bacha
;
Weffort, Denise
;
Adolpho, Leticia Faustino
;
Gomes, Maria Paula Oliveira
;
Oliveira, Fabiola Singaretti
;
Almeida, Adriana Luisa Goncalves
;
Beloti, Marcio Mateus
;
Rosa, Adalberto Luiz
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Life Sciences; v. 340, p. 12-pg., 2024-01-29. |
| Resumo | |
Aims: Cell therapy utilizing mesenchymal stem cells (MSCs) from healthy donors (HE-MSCs) is a promising strategy for treating osteoporotic bone defects. This study investigated the effects of interaction between HEMSCs and MSCs from osteoporotic donors (ORX-MSCs) on osteoblast differentiation of MSCs and of HE-MSCs on bone formation in calvarial defects of osteoporotic rats. Materials and methods: Osteoporosis was induced by orchiectomy (ORX) and its effects on the bone were evaluated by femur microtomography (mu CT) and osteoblast differentiation of bone marrow MSCs. HE- and ORX-MSCs were cocultured, and osteoblast differentiation was evaluated using genotypic and phenotypic parameters. HEMSCs were injected into the calvarial defects of osteoporotic rats, and bone formation was evaluated by mu CT, histology, and gene expression of osteoblast markers. Key findings: ORX-induced osteoporosis was revealed by reduced bone morphometric parameters and osteoblast differentiation in ORX-MSCs. HE-MSCs partially recovered the osteogenic potential of ORX-MSCs, whereas HEMSCs were mildly affected by ORX-MSCs. Additionally, the bone morphogenetic protein and wingless-related integration site signaling pathway components were similarly modulated in cocultures involving ORX-MSCs. HE-MSCs induced meaningful bone formation, highlighting the effectiveness of cell therapy even in osteoporotic bones. Significance: These results provide new perspectives on the development of cell-based therapies to regenerate bone defects in patients with disorders that affect bone tissue. (AU) | |
| Processo FAPESP: | 17/12622-7 - Terapia celular: potencial de células-tronco mesenquimais, VEGF-A e BMP-9 para regenerar tecido ósseo |
| Beneficiário: | Adalberto Luiz Rosa |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 18/13290-0 - Terapia celular: avaliação do efeito de células-tronco mesenquimais para regenerar tecido ósseo de ratos com Osteoporose, Diabetes Mellitus ou Hipertensão Arterial |
| Beneficiário: | Alann Thaffarell Portilho de Souza |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 19/08048-9 - Efeito da mutação de Ggps1 nas funções de células ósseas in vitro e na fragilidade diafisária in vivo usando camundongos após cirurgia de ovariectomia e expostos ao tratamento de longa duração com bifosfonatos contendo nitrogênio |
| Beneficiário: | Alann Thaffarell Portilho de Souza |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado |