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Bone Resorption in Apical Periodontitis Enhanced by Cigarette Smoke Inhalation: Histometric, Immunohistochemical, and Microtomographic Analysis in Rats

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Autor(es):
Vasques, Ana Maria Veiga ; da Silva, Ana Claudia Rodrigues ; Bueno, Carlos Roberto Emerenciano ; Duarte, Marco Antonio Hungaro ; Ervolino, Edilson ; Cintra, Luciano Tavares Angelo ; Dezan Jr, Eloi
Número total de Autores: 7
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF ENDODONTICS; v. 50, n. 4, p. 6-pg., 2024-03-26.
Resumo

Introduction: This study evaluated the effects of cigarette smoke inhalation (CSI) on apical periodontitis (AP) induced in rats by histometric, immunohistochemical, and microtomographic analysis. Methods: A total of 32 male Wistar rats were divided into 4 experimental groups ( n = 8): control, CSI, AP, and CSI + AP. Rats in the CSI and CSI + AP groups inhaled cigarette smoke by remaining inside a smoking chamber for 8 minutes 3 times a day for 50 days. After 20 days of smoke inhalation, rats in the AP and CSI + AP groups had the pulp of their first right lower molar exposed to induce AP. Blood was collected on day 50 to evaluate nicotine and serum cotinine levels. The animals ' mandibles were removed for histologic processing to evaluate bone resorption by histometric, immunohistochemical (receptor activator of nuclear factor kappa B ligand/osteoprotegerin), and microtomographic analysis. The Student t test was applied. Results: Histometric analysis showed a larger area of bone resorption ( P < .05) and microtomographic analysis found greater resorption volume ( P < .001) for the CSI + AP group compared with the AP group. The CSI + AP group presented a high RANKL immunostaining pattern compared with the AP group ( P < .001). Conclusions: CSI increased bone resorption caused by AP. (AU)

Processo FAPESP: 20/06310-5 - Influência da inalação de fumaça de cigarro no desenvolvimento da periodontite apical: análise do perfil inflamatório local e sistêmico em ratos Wistar
Beneficiário:Eloi Dezan Junior
Modalidade de apoio: Auxílio à Pesquisa - Regular