| Texto completo | |
| Autor(es): Mostrar menos - |
Costa, Juliana N. Y.
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Pimentel, Gabriel J. C.
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Poker, Julia A.
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Merces, Leandro
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Paschoalino, Waldemir J.
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Vieira, Luis C. S.
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Castro, Ana C. H.
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Alves, Wendel A.
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Ayres, Lucas B.
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Kubota, Lauro T.
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Santhiago, Murilo
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Garcia, Carlos D.
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Piazzetta, Maria H. O.
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Gobbi, Angelo L.
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Shimizu, Flavio M.
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Lima, Renato S.
Número total de Autores: 16
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| Tipo de documento: | Artigo Científico |
| Fonte: | ADVANCED HEALTHCARE MATERIALS; v. 13, n. 11, p. 14-pg., 2024-02-22. |
| Resumo | |
Multiplexing is a valuable strategy to boost throughput and improve clinical accuracy. Exploiting the vertical, meshed design of reproducible and low-cost ultra-dense electrochemical chips, the unprecedented single-response multiplexing of typical label-free biosensors is reported. Using a cheap, handheld one-channel workstation and a single redox probe, that is, ferro/ferricyanide, the recognition events taking place on two spatially resolved locations of the same working electrode can be tracked along a single voltammetry scan by collecting the electrochemical signatures of the probe in relation to different quasi-reference electrodes, Au (0 V) and Ag/AgCl ink (+0.2 V). This spatial isolation prevents crosstalk between the redox tags and interferences over functionalization and binding steps, representing an advantage over the existing non-spatially resolved single-response multiplex strategies. As proof of concept, peptide-tethered immunosensors are demonstrated to provide the duplex detection of COVID-19 antibodies, thereby doubling the throughput while achieving 100% accuracy in serum samples. The approach is envisioned to enable broad applications in high-throughput and multi-analyte platforms, as it can be tailored to other biosensing devices and formats. Meshed ultra-dense chips provide the single-response duplexing of label-free electrochemical biosensors. Distinguishable signatures are achieved by interrogating the same redox probe in spatially resolved areas of the same working electrode relative to distinct quasi-reference electrodes, Au and Ag/AgCl. This spatial isolation prevents crosstalk between probes and interferences over functionalization/binding steps, representing an advantage over existing non-spatially resolved single-response multiplex strategies. image (AU) | |
| Processo FAPESP: | 22/04397-1 - Chips eletroquímicos verticais em malha ultradensos para diagnósticos multiplexados a partir de uma única resposta |
| Beneficiário: | Juliana Naomi Yamauti Costa |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 23/02080-3 - Sensores eletroquímicos verticais ultradensos para análise da bactéria Staphylococcus aureus com alta capacidade de testagem |
| Beneficiário: | Renato Sousa Lima |
| Modalidade de apoio: | Bolsas no Exterior - Pesquisa |