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Pereira, Bruna Pinheiro ; Silva, Alessandra Oliveira ; Awata, Wanessa Mayumi Carvalho ; Pimenta, Gustavo Felix ; Ribeiro, Jessyca Milene ; Almeida, Carolina Aparecida de Faria ; Antonietto, Carla Renata Kitanishi ; dos Reis, Luis Felipe Cunha ; Esteves, Alessandra ; Torres, Larissa Helena Lobo ; Paula, Fernanda Borges de Araujo ; Ruginsk, Silvia Graciela ; Tirapelli, Carlos Renato ; Rizzi, Ellen ; Ceron, Carla Speroni

• Tipo de documento: Artigo Científico
• Fonte: Cell Biochemistry and Function; v. 42, n. 7, p. 12-pg., 2024-09-01.

Resumo

In the present study, we investigated whether curcumin administration would interfere with the main renal features of L-NAME-induced hypertension model. For this purpose, we conducted both in vitro and in vivo experiments to evaluate renal indicators of inflammation, oxidative stress, and metalloproteinases (MMPs) expression/activity. Hypertension was induced by L-NAME (70 mg/kg/day), and Wistar rats from both control and hypertensive groups were treated with curcumin (50 or 100 mg/kg/day; gavage) or vehicle for 14 days. Blood and kidneys were collected to determine serum creatinine levels, histological alterations, oxidative stress, MMPs expression and activity, and ED1 expression.L-NAME increased blood pressure, but both doses of curcumin treatment reduced these values. L-NAME treatment increased creatinine levels, glomeruli area, Bowman's space, kidney MMP-2 activity, as well as MMP-9 and ED1 expression, and reduced the number of glomeruli. Curcumin treatment prevented the increase in creatinine levels, MMP-2 activity, and reduced MMP-2, MMP-9, ED1, and superoxide levels, as well as increased superoxide dismutase activity and partially prevented glomeruli alterations. Moreover, curcumin directly inhibited MMP-2 activity in vitro. Thus, our main findings demonstrate that curcumin reduced L-NAME-induced hypertension and renal glomerular alterations, inhibited MMP-2 and MMP-9 expression/activity, and reduced oxidative stress and inflammatory processes, which may indirectly impact hypertension-induced renal outcomes. (AU)

• Processo FAPESP: 19/25189-5 - Efeito do consumo crônico de etanol sobre a ação modulatória do tecido adiposo perivascular: papel da angiotensina II
• Beneficiário: Carlos Renato Tirapelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular