| Texto completo | |
| Autor(es): |
dos Santos, Danilo Martins
;
Moon, Jae-I.
;
Kim, Da-Seul
;
Bassous, Nicole Joy
;
Marangon, Crisiane Aparecida
;
Campana-Filho, Sergio Paulo
;
Correa, Daniel Souza
;
Kang, Min-Ho
;
Kim, Woo-Jin
;
Shin, Su Ryon
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ACS NANO; v. 18, n. 35, p. 22-pg., 2024-08-20. |
| Resumo | |
Periodontitis, a prevalent chronic inflammatory disease caused by bacteria, poses a significant challenge to current treatments by merely slowing their progression. Herein, we propose an innovative solution in the form of hierarchical nanostructured 3D printed bilayer membranes that serve as dual-drug delivery nanoplatforms and provide scaffold function for the regeneration of periodontal tissue. Nanocomposite hydrogels were prepared by combining lipid nanoparticle-loaded grape seed extract and simvastatin, as well as chitin nanocrystals, which were then 3D printed into a bilayer membrane that possesses antimicrobial properties and multiscale porosity for periodontal tissue regeneration. The constructs exhibited excellent mechanical properties by adding chitin nanocrystals and provided a sustained release of distinct drugs over 24 days. We demonstrated that the bilayer membranes are cytocompatible and have the ability to induce bone-forming markers in human mesenchymal stem cells, while showing potent antibacterial activity against pathogens associated with periodontitis. In vivo studies further confirmed the efficacy of bilayer membranes in enhancing alveolar bone regeneration and reducing inflammation in a periodontal defect model. This approach suggests promising avenues for the development of implantable constructs that not only combat infections, but also promote the regeneration of periodontal tissue, providing valuable insights into advanced periodontitis treatment strategies. (AU) | |
| Processo FAPESP: | 18/22214-6 - Rumo à convergência de tecnologias: de sensores e biossensores à visualização de informação e aprendizado de máquina para análise de dados em diagnóstico clínico |
| Beneficiário: | Osvaldo Novais de Oliveira Junior |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 19/22799-7 - Explorando o potencial de nanopartículas de biopolímeros com extratos de frutas para controle de patógenos alimentares |
| Beneficiário: | Crisiane Aparecida Marangon |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 17/20973-4 - Nanofibras eletrofiadas coaxiais à base de quitosana para a liberação controlada de antibióticos e aplicações no tratamento de lesões periodontais |
| Beneficiário: | Danilo Martins dos Santos |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |