Autor(es)

Fortes, Bruna Nakanishi ; Wirth, Fernanda ; dos Santos, Aline Martins ; Chorilli, Marlus ; Freitas, Vanessa Morais ; Farias, Jennifer ; Chambergo, Felipe S. ; Dantas, Viviane Abreu Nunes C. ; Ishida, Kelly

• Tipo de documento: Artigo Científico
• Fonte: FUTURE MICROBIOLOGY; v. N/A, p. 14-pg., 2024-07-11.

Resumo

Aim: This work aims to standardize the three-dimensional hydroxyethyl-alginate-gelatin (HAG) scaffold as a model to evaluate Aspergillus fumigatus biofilm and antifungal treatments. Methods: The scaffold was characterized by physical, rheological and microscopic analyses; the antibiofilm action was evaluated by determination of cfu and metabolic activity. Results: The scaffold was non-toxic showing stability in aqueous media, swelling capacity, elasticity and had homogeneously distributed pores averaging 190 mu m. The A. fumigatus biofilm established itself very well on the scaffold and treatment with amphotericin B and voriconazole reduced viable cells and metabolic activity. Conclusion: The HAG scaffold proved to be a model to mimic lung parenchyma, suitable for establishing a 3D biofilm culture of A. fumigatus and evaluating the efficacy of antifungals. (AU)

• Processo FAPESP: 20/11486-5 - Desenvolvimento de uma estrutura polimérica tridimensional para avaliar os efeitos de antifúngicos na interação com Aspergillus fumigatus
• Beneficiário: Bruna Nakanishi Fortes
• Modalidade de apoio: Bolsas no Brasil - Mestrado