Evaluation of Hydrothermal and Alkaline Pretreatment Routes for Xylooligosaccharides Production from Sugar Cane Bagasse Using Different Combinations of Recombinant Enzymes

Autor(es)

Capetti, Caio Cesar de Mello ; Pellegrini, Vanessa de Oliveira Arnoldi ; Vacilotto, Milena Moreira ; Curvelo, Antonio Aprigio da Silva ; Falvo, Mauricio ; Guimaraes, Francisco Eduardo Gontijo ; Ontanon, Ornella M. ; Campos, Eleonora ; Polikarpov, Igor

• Tipo de documento: Artigo Científico
• Fonte: Food and Bioprocess Technology; v. 17, n. 7, p. 13-pg., 2023-10-18.

Resumo

Xylan is the most abundant constituent of hemicellulose fraction of lignocellulosic biomass. Short xylooligosaccharides (XOS), obtained via xylan hydrolysis, have well-known prebiotic and antioxidant properties that are beneficial for human and animal health. In this study, two alternative pretreatment strategies (alkali and hydrothermal) and three different enzymes were applied for enzymatic XOS production from sugarcane bagasse. The enzymatic hydrolysis was performed with nine different combinations of recombinant endo-xylanases from GH11 and GH10 families and GH11 xylobiohydrolase. Hydrothermal pretreatment followed by optimized enzymatic hydrolysis yielded up to 96 +/- 1 mg of XOS per gram of initial biomass, whereas enzymatic hydrolysis of alkali-pretreated sugarcane bagasse rendered around 47.6 +/- 0.2 mg/g. For both alkali and hydrothermal routes, the maximum yields of short-length XOS were obtained using the GH10 xylanase alone. Furthermore, differences in XOS profiles obtained by controlled mixtures of the enzymes have been evaluated. For both routes, the best yields of short-length XOS were obtained using the GH10 xylanase alone, which is consistent with the notion that sugarcane xylan substitutions partially hinder GH11 xylanase activity. The results presented here show that a green and cost-effective hydrothermal pretreatment path for xylooligosaccharides production, rendered considerably better XOS yields. (AU)

• Processo FAPESP: 19/10942-0 - Produção heteróloga e caracterização da xilanases de famílias GH10 e GH11 e avaliação das suas aplicações na degradação de xilano e obtenção de xilooligossacarídeos a partir da biomassa lignocelulósica
• Beneficiário: Caio Cesar de Mello Capetti
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 21/08780-1 - Degradação enzimática de exopolissacharídeos de biofilmes microbianos: abordagens de biofísica estrutural, biotecnologia molecular e bioquímica sintética em estudos de CAZymes em busca de novas ferramentas enzimáticas para tratamentos antimicrobianos
• Beneficiário: Igor Polikarpov
• Modalidade de apoio: Auxílio à Pesquisa - Temático