Hybrid crystalline bioparticles with nanochannels encapsulating acemannan from Aloe vera: Structure and interaction with lipid membranes

Autor(es)

Madrid, Rafael R. M. ; Mathews, Patrick D. ; Pramanik, Shreya ; Mangiarotti, Agustin ; Fernandes, Rodrigo ; Itri, Rosangela ; Dimova, Rumiana ; Mertins, Omar

• Tipo de documento: Artigo Científico
• Fonte: Journal of Colloid and Interface Science; v. 673, p. 13-pg., 2024-06-14.

Resumo

Smart nanocarrier-based bioactive delivery systems are a current focus in nanomedicine for allowing and boosting diverse disease treatments. In this context, the design of hybrid lipid-polymer particles can provide structure-sensitive features for tailored, triggered, and stimuli-responsive devices. In this work, we introduce hybrid cubosomes that have been surface-modified with a complex of chitosan-N-arginine and alginate, making them pH-responsive. We achieved high-efficiency encapsulation of acemannan, a bioactive polysaccharide from Aloe vera, within the nanochannels of the bioparticle crystalline structure and demonstrated its controlled release under pH conditions mimicking the gastric and intestinal environments. Furthermore, an acemannan-induced phase transition from Im3m cubic symmetry to inverse hexagonal HII phase enhances the bioactive delivery by compressing the lattice spacing of the cubosome water nanochannels, facilitating the expulsion of the encapsulated solution. We also explored the bioparticle interaction with membranes of varying curvatures, revealing thermodynamically driven affinity towards high-curvature lipid membranes and inducing morphological transformations in giant unilamellar vesicles. These findings underscore the potential of these structure -responsive, membrane -active smart bioparticles for applications such as pH -triggered drug delivery platforms for the gastrointestinal tract, and as modulators and promoters of cellular internalization. (AU)

• Processo FAPESP: 21/00971-2 - Nano cubossoma de fase cristalina e funcionalizado com biopolímeros: desenvolvimento como carreador de fármaco e estudos in vivo em zebrafish (Danio rerio)
• Beneficiário: Omar Mertins
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 22/12376-4 - Desenvolvimento de tratamento parasitário em peixes com nanopartículas: uma abordagem tecnológico-aplicativa
• Beneficiário: Patrick Mathews Delgado
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores