Satellitome analysis on the pale-breasted thrush Turdus leucomelas (Passeriformes; Turdidae) uncovers the putative co-evolution of sex chromosomes and satellite DNAs

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Souza, Guilherme Mota ; Kretschmer, Rafael ; Toma, Gustavo Akira ; de Oliveira, Alan Moura ; Deon, Geize Aparecida ; Setti, Princia Grejo ; dos Santos, Rodrigo Zeni ; Goes, Caio Augusto Gomes ; Garnero, Analia Del Valle ; Gunski, Ricardo Jose ; de Oliveira, Edivaldo Herculano Correa ; Porto-Foresti, Fabio ; Liehr, Thomas ; Utsunomia, Ricardo ; Cioffi, Marcelo de Bello

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 14, n. 1, p. 14-pg., 2024-09-04.

Resumo

Do all birds' sex chromosomes follow the same canonical one-way direction of evolution? We combined cytogenetic and genomic approaches to analyze the process of the W chromosomal differentiation in two selected Passeriform species, named the Pale-breasted Thrush Turdus leucomelas and the Rufous-bellied thrush T. rufiventris. We characterized the full catalog of satellite DNAs (satellitome) of T. leucomelas, and the 10 TleSatDNA classes obtained together with 16 microsatellite motifs were in situ mapped in both species. Additionally, using Comparative Genomic Hybridization (CGH) assays, we investigated their intragenomic variations. The W chromosomes of both species did not accumulate higher amounts of both heterochromatin and repetitive sequences. However, while T. leucomelas showed a heterochromatin-poor W chromosome with a very complex evolutionary history, T. rufiventris showed a small and partially heterochromatic W chromosome that represents a differentiated version of its original autosomal complement (Z chromosome). The combined approach of CGH and sequential satDNA mapping suggest the occurrence of a former W-autosomal translocation event in T. leucomelas, which had an impact on the W chromosome in terms of sequence gains and losses. At the same time, an autosome, which is present in both males and females in a polymorphic state, lost sequences and integrated previously W-specific ones. This putative W-autosomal translocation, however, did not result in the emergence of a multiple-sex chromosome system. Instead, the generation of a neo-W chromosome suggests an unexpected evolutionary trajectory that deviates from the standard canonical model of sex chromosome evolution. (AU)

• Processo FAPESP: 23/00955-2 - Integrando citogenética e genômica no estudo das vias de diferenciação dos cromossomos sexuais. Parte I
• Beneficiário: Marcelo de Bello Cioffi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 22/14584-3 - DNAs satélites e a diferenciação cariotípica e dos cromossomos sexuais ZZ/ZW em aves. Parte IV. O gavião-real (Harpia harpyja) como modelo
• Beneficiário: Guilherme Mota Souza
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 22/00427-3 - Origem e diferenciação do sistema de cromossomos sexuais múltiplos X1X2Y: análises do Satelitoma em espécies de peixes, répteis e mamíferos
• Beneficiário: Gustavo Akira Toma
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto