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Molecular detection of Chlamydiales organisms in ruminant abortions in Brazil

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Pinto, Ana Paula Vieira ; Teixeira, Wanderson Sirley Reis ; Malossi, Camila Dantas ; Possebon, Fabio Sossai ; Henker, Luan Cleber ; Lorenzett, Marina Paula ; Pavarini, Saulo Petinatti ; Driemeier, David ; Casagrande, Renata Assis ; Araujo Junior, Joao Pessoa ; Megid, Jane ; de Mioni, Mateus Souza Ribeiro
Número total de Autores: 12
Tipo de documento: Artigo Científico
Fonte: Brazilian Journal of Microbiology; v. N/A, p. 8-pg., 2024-12-02.
Resumo

Infections caused by the Chlamydiales order are highly prevalent worldwide and have a well-known association with reproductive and non-reproductive disorders in livestock and humans. This study aimed to identify Chlamydiales organisms in ruminant abortions using real-time polymerase chain reaction (qPCR) in samples collected from three Brazilian states: Rio Grande do Sul, Santa Catarina, and S & atilde;o Paulo. The Chlamydiales order was found in 7.8% (18/231) of the samples, including 10 bovine and 8 ovine samples. For the Chlamydiaceae family, the positivity was 2.2% (5/231), with detection in one bovine and two ovine fetuses from Rio Grande do Sul, one ovine fetus from Santa Catarina, and one bovine from S & atilde;o Paulo. Chlamydiales positive samples detection with Ct value lower than 35 were subjected to Sanger sequencing and phylogenetic analysis, which revealed the presence of an organism belonging to the Waddliaceae family in a bovine fetus and two Simkaneceae bacteria in ovine fetuses. This study is the first report of Chlamydia-like organisms in ruminant abortions in Brazil, highlighting the importance of conducting further surveys to evaluate their role as abortifacient pathogens in the country. (AU)

Processo FAPESP: 21/02489-3 - Avaliação molecular do envolvimento de Chlamydiales, Chlamydophila abortus e Chlamydia-Like em abortamentos de ruminantes.
Beneficiário:Ana Paula Vieira Pinto
Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 19/05300-9 - Isolamento, caracterização e sequenciamento genético de estirpes de Coxiella burnetii do Brasil
Beneficiário:Mateus de Souza Ribeiro Mioni
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado