Peptides from Galleria mellonella against Cryptococcus spp: toxicity in three-dimensional cell cultures and G. mellonella

Autor(es)

Gualque, Marcos William De Lima ; Vaso, Carolina Orlando ; dos Santos, Kelvin Sousa ; Galeane, Mariana Cristina ; Gomes, Paulo Cesar ; Palma, Mario Sergio ; Giannini, Maria Jose Soares Mendes ; Moroz, Andrei ; Almeida, Ana Marisa Fusco

• Tipo de documento: Artigo Científico
• Fonte: FUTURE MICROBIOLOGY; v. N/A, p. 11-pg., 2024-11-18.

Resumo

Aim: This work aimed to test peptides against the planktonic and biofilm form of Cryptococcus spp. and in vitro toxicity using three-dimensional (3D) cells characterized and evaluate in vivo toxicity in Galleria mellonella.Materials & methods: Susceptibility tests were conducted on the planktonic form and biofilm formation. The toxicity of the peptides was evaluated in lung and brain cells in monolayer (2D) and 3D mono- and co-culture, in addition to in vivo analysis with G. mellonella.Results: Susceptibility values ranged from 31.25 to over 250 mu g/ml with a fungicidal profile. Regarding toxicity, the PepM2 peptide was not toxic in 3D culture (500 mu g/ml). G. mellonella, showed a survival rate of more than 85% In assays with brain and lung cell lines, concentrations ranged from 4 x 104 to 4 x 103 cells/well for brain cells and 1 x 103 cells/well for lung cells. Cocultures used 1 x 105 brain and 1 x 103 lung cells.Conclusion: This study shows that the peptides have great potential against cryptococcosis, and all spheroids were characterized as having a spheroidal and compact structure. (AU)

• Processo FAPESP: 22/15826-0 - Do desenvolvimento de modelos pulmonares humanos tridimensionais de infecção à evolução de modelos pulmonares humanos funcionais para o estudo da interação com biofilme de Histoplasma capsulatum
• Beneficiário: Ana Marisa Fusco Almeida
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 21/14839-9 - Desenvolvimento de co-cultura de esferoides pulmonares e cerebrais e organoide cerebral para a avaliação da interação de Cryptococcus sp. e estudo da atividade antifúngica de peptídeos derivados de Galleria mellonella
• Beneficiário: Marcos William de Lima Gualque
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 20/15586-4 - Estabelecimento de um modelo tridimensional para determinação da eficácia e segurança farmacológica de derivados de nitrofuranos e indólicos e avaliação da infecção de Histoplasma capsulatum
• Beneficiário: Carolina Orlando Vaso
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto