| Texto completo | |
| Autor(es): |
de Freitas, Ana Claudia
;
Reolon, Henrique G.
;
Abduch, Natalya G.
;
Baldi, Fernando
;
Silva, Rafael M. O.
;
Lourenco, Daniela
;
Fragomeni, Breno O.
;
Paz, Claudia C. P.
;
Stafuzza, Nedenia B.
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BMC Genomics; v. 25, n. 1, p. 20-pg., 2024-11-13. |
| Resumo | |
BackgroundHeat stress has deleterious effects on physiological and performance traits in livestock. Within this context, using tropically adapted cattle breeds in pure herds or terminal crossbreeding schemes to explore heterosis is attractive for increasing animal production in warmer climate regions. This study aimed to identify biological processes, pathways, and potential biomarkers related to thermotolerance in Caracu, a tropically adapted beef cattle breed, by proteomic analysis of blood plasma. To achieve this goal, 61 bulls had their thermotolerance evaluated through a heat tolerance index. A subset of 14 extreme animals, including the seven most thermotolerant (HIGH group) and the seven least thermotolerant (LOW group), had their blood plasma samples used for proteomic analysis by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The differentially regulated proteins detected between HIGH and LOW groups were used to perform functional enrichment analysis and a protein-protein interaction network analysis.ResultsA total of 217 proteins were detected only in the HIGH thermotolerant group and 51 only in the LOW thermotolerant group. In addition, 81 and 87 proteins had significantly higher and lower abundancies in the HIGH group, respectively. Regarding proteins with the highest absolute log-fold change values, we highlighted those encoded by DUSP5, IGFALS, ROCK2, RTN4, IRAG1, and NNT genes based on their functions. The functional enrichment analysis detected several biological processes, molecular functions, and pathways related to cellular responses to stress, immune system, complement system, and hemostasis in both HIGH and LOW groups, in addition to terms and pathways related to lipids and calcium only in the HIGH group. Protein-protein interaction (PPI) network revealed as important nodes many proteins with roles in response to stress, hemostasis, immune system, inflammation, and homeostasis. Additionally, proteins with high absolute log-fold change values and proteins detected as essential nodes by PPI analysis highlighted herein are potential biomarkers for thermotolerance, such as ADRA1A, APOA1, APOB, APOC3, C4BPA, CAT, CFB, CFH, CLU, CXADR, DNAJB1, DNAJC13, DUSP5, FGA, FGB, FGG, HBA, HBB, HP, HSPD1, IGFALS, IRAG1, KNG1, NNT, OSGIN1, PROC, PROS1, ROCK2, RTN4, RYR1, TGFB2, VLDLR, VTN, and VWF.ConclusionsIdentifying potential biomarkers, molecular mechanisms and pathways that act in response to heat stress in tropically adapted beef cattle contributes to developing strategies to improve performance and welfare traits in livestock under tropical climates. (AU) | |
| Processo FAPESP: | 20/03699-9 - Fenotipagem para termotolerância, eficiência alimentar e resistência a carrapatos em bovinos Caracu |
| Beneficiário: | Natalya Gardezani Abduch |
| Modalidade de apoio: | Bolsas no Brasil - Programa Capacitação - Treinamento Técnico |
| Processo FAPESP: | 19/10438-0 - Análise integrada do transcriptoma e proteoma para identificação de biomarcadores para eficiência alimentar, termotolerância e resistência à carrapato em bovinos Caracu |
| Beneficiário: | Nedenia Bonvino Stafuzza |
| Modalidade de apoio: | Bolsas no Brasil - Jovens Pesquisadores |
| Processo FAPESP: | 19/17251-2 - Banco de dados fenotípicos para características de adaptabilidade e produção em bovinos Caracu |
| Beneficiário: | Natalya Gardezani Abduch |
| Modalidade de apoio: | Bolsas no Brasil - Programa Capacitação - Treinamento Técnico |