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Vergara-Meza, Jose Gabriel ; Brilhante, Andreia Fernandes ; Valente, Vera da Costa ; Villalba-Aleman, Evaristo ; Ortiz, Paola Andrea ; de Oliveira, Sueli Cosmiro ; Cavalcante, Maxdelles Rodrigues ; Juliao, Genimar Reboucas ; Goncalves Pinto, Maria Carmelinda ; Valente, Sebastiao Aldo ; Camargo, Erney Plesmann ; Geraldes Teixeira, Marta Maria

• Tipo de documento: Artigo Científico
• Fonte: PARASITOLOGIA; v. 2, n. 4, p. 16-pg., 2022-12-01.

Resumo

Acute Chagas disease (ACD) caused by Trypanosoma cruzi has emerged as a major food-borne disease in Brazilian Amazonia. For the first time, we characterized an outbreak of orally acquired ACD in Acre, in the forest community of Seringal Miraflores, affecting 13 individuals who shared the pulp of a & ccedil;ai palm berries: 11 adults and two children (one newborn), all diagnosed by thick-drop blood smears. The fluorescent fragment length barcoding method, which simultaneously identifies species/genotypes of trypanosomes in blood samples, uncovered an unprecedented genetic diversity in patients from a single outbreak of ACD: T. cruzi TcI in all patients, mostly concomitantly with the non-pathogenic Trypanosoma rangeli of genotypes TrA or TrB, and TcI, TcIV, and TrB in the child. The patients presented persistent fever, asthenia, myalgia, edema of the face and lower limbs, hepatosplenomegaly and, rarely, cardiac arrhythmia. The clinical symptoms were not correlated to gender, age, or to trypanosome species and genotypes. The inferred SSU rRNA phylogenetic analyses of trypanosomes from humans, triatomines and sylvatic hosts included the first sequences of T. cruzi and T. rangeli from humans in southwestern (Acre and Rond & ocirc;nia) Amazonia, and the first TcI/TcIV sequences from Rhodnius spp. from Acre. The sylvatic transmission cycles of genetically different trypanosomes in landscapes changed by deforestation for human settlements and increasing a & ccedil;ai production is a novel scenario favoring trypanosome transmission to humans in Acre. (AU)

• Processo FAPESP: 16/07487-0 - Ampliação e codificação (DNA barcoding) da coleção de culturas de tripanossomatídeos da USP (TCC-USP) e sua utilização em estudos filogenéticos e taxonômicos
• Beneficiário: Erney Felicio Plessmann de Camargo
• Modalidade de apoio: Auxílio à Pesquisa - Regular