Autor(es)

Garcia-Fossa, Fernanda ; Cruz, Mario Costa ; Haghighi, Marzieh ; de Jesus, Marcelo Bispo ; Singh, Shantanu ; Carpenter, Anne E. ; Cimini, Beth A.

• Tipo de documento: Artigo Científico
• Fonte: CURRENT PROTOCOLS; v. 3, n. 3, p. 21-pg., 2023-03-01.

Resumo

Image-based profiling quantitatively assesses the effects of perturbations on cells by capturing a breadth of changes via microscopy. Here, we provide two complementary protocols to help explore and interpret data from image-based profiling experiments. In the first protocol, we examine the similarity among perturbed cell samples using data from compounds that cluster by their mechanisms of action. The protocol includes steps to examine feature-driving differences between samples and to visualize correlations between features and treatments to create interpretable heatmaps using the open-source web tool Morpheus. In the second protocol, we show how to interactively explore images together with the numerical data, and we provide scripts to create visualizations of representative single cells and image sites to understand how changes in features are reflected in the images. Together, these two tutorials help researchers interpret image-based data to speed up research. (c) 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Exploratory analysis of profile similarities and driving featuresBasic Protocol 2: Image and single-cell visualization following profile interpretation (AU)

• Processo FAPESP: 19/24033-1 - Alterações sutis desencadeadas por nanopartículas em células analisadas individualmente através de perfil multiparamétrico baseada em imagem
• Beneficiário: Fernanda Garcia Fóssa
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 22/01483-4 - Alterações sutis desencadeadas por nanopartículas em células analisadas individualmente através de perfil multiparamétrico baseada em imagem
• Beneficiário: Fernanda Garcia Fóssa
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 20/01218-3 - Nanotoxicidade analisada individualmente através de perfil multiparamétrico baseada em imagem
• Beneficiário: Marcelo Bispo de Jesus
• Modalidade de apoio: Auxílio à Pesquisa - Regular