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Properties and kinetic behavior of free and immobilized laccase from Oudemansiella canarii: Emphasis on the effects of NaCl and Na2SO4 on catalytic activities

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Autor(es):
Backes, Emanueli ; Alnoch, Robson Carlos ; Castoldi, Rafael ; Souza, Cristina Giatti Marques de ; Kato, Camila Gabriel ; Peralta, Rosely Aparecida ; Moreira, Regina de Fatima Peralta Muniz ; Polizeli, Maria de Lourdes T. M. ; Bracht, Adelar ; Peralta, Rosane Marina
Número total de Autores: 10
Tipo de documento: Artigo Científico
Fonte: International Journal of Biological Macromolecules; v. 281, p. 14-pg., 2024-10-19.
Resumo

Studies have highlighted the great potential of Oudemansiella canarii laccase in degrading synthetic dyes for reducing their toxicity. Immobilization of enzymes improves usability in degradation processes and the present work succeeded in immobilizing this laccase onto MANAE-agarose. Immobilization improved pH, thermal, and storage stabilities. Both, free and immobilized enzymes presented Michaelis-Menten kinetics with the substrate 2,2 '-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) with Km values of 0.056 +/- 0.003 and 0.195 +/- 0.022 mM, respectively. Immobilization increased V max 1.27-fold. NaCl caused incomplete (hyperbolic) inhibition, which was satisfactorily described by the one-substrate one-modifier mechanism. Immobilization reduced the maximal inhibition by NaCl from 80.2 to 55.7 %. The effect of Na2SO4 was predominantly stimulation, but inhibition of the free enzyme occurred at high substrate concentrations. Stimulation of the immobilized enzyme by Na2SO4 was much more pronounced. It strongly depended on the substrate concentration and was much stronger (up to 300 %) at low substrate concentrations. The combined effects of substrate and sulfate on the immobilized laccase could be satisfactorily described by the one-substrate one-modifier mechanism. The modified response of the immobilized O. canarii laccase to NaCl and Na2SO4 considerably favors its use as a tool in bioremediation processes because environmental contamination by salts frequently represents a strong operational challenge. (AU)

Processo FAPESP: 23/09627-8 - Formulação de consórcios enzimáticos "tailor-made" para a degradação da parede celular de variedades de cana-de-açúcar
Beneficiário:Robson Carlos Alnoch
Modalidade de apoio: Bolsas no Brasil - Programa Fixação de Jovens Doutores