| Texto completo | |
| Autor(es): Mostrar menos - |
de Almeida, Maria Alice
;
Haupenthal, Laura Gabrielli
;
Silva, Amanda Nespolo
;
Schneider, Gabriela Melendes
;
Rosa, Paola Maria da Silva
;
de Andrade, Andre Furugenesar
;
Silva, Luciano Andrade
;
Meirelles, Flavio Vieira
;
da Silveira, Juliano Coelho
;
Perecin, Felipe
;
Alves, Marra Bianchi Rodrigues
Número total de Autores: 11
|
| Tipo de documento: | Artigo Científico |
| Fonte: | CRYOBIOLOGY; v. 115, p. 12-pg., 2024-05-25. |
| Resumo | |
While cryopreservation of cauda epididymal sperm (SpCau) allows the preservation of post-mortem bulls' gametes, the process triggers sperm damage. Although improving post-thaw sperm quality, using egg yolk extenders (EY) raises biosafety concerns which forces the use of EY-free extenders (EYFE). Since EYFE are less efficient in preserving post-thaw sperm quality, a strategy for ejaculated sperm (SpEj) frozen with EYFE is to add an Equilibrium Time (ET) step period to the cryopreservation process. However, the ET effect on the quality of SpCau cryopreserved in EYFE remains unknown. Distinct from SpEJ, SpCau physiologically displays cytoplasmic droplets (CDs) in the flagellum that may benefit cell exchange during ET. We hypothesized that using ET in SpCau cryopreserved with EYFE impacts sperm morphofunctional features, CD area, and in vitro fertility ability. Extender nanoparticles were also assessed. Following collection from the cauda epididymis of six Nellore bulls by retrograde flow, SpCau were cryopreserved in EYFE BoviFree (R) (Minitube, Germany) using three ET protocols: ET0 (no-ET); ET2.5 (2.5 h-ET); and ET5 (5 h-ET). SpCau from ET2.5 and ET5 showed a higher (P <= 0.05) percentage of motility and integrity of plasma and acrosome membranes and a smaller (P <= 0.05) distal CD area. There are no differences in sperm abnormalities, oxidative stress, capacitation-like events, and in vitro fertility ability. However, a better sperm recovery was found after Percoll (R) selection for ET2.5 and ET5. Interestingly, the number of nanoparticles in the extender decreased in post-thawed samples. In conclusion, an ET of 2.5 or 5 h is required for an efficient SpCau cryopreservation using an EYFE. (AU) | |
| Processo FAPESP: | 19/23685-5 - Interações epidídimo-espermatozoide em condições de estresse térmico: efeitos na contribuição paterna para o desenvolvimento embrionário |
| Beneficiário: | Maíra Bianchi Rodrigues Alves |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 21/08759-2 - Mensagens armazenadas no gameta masculino bovino: efeitos do estresse térmico, papel das interações epidídimo-espermatozoide e contribuição paterna ao desenvolvimento embrionário |
| Beneficiário: | Felipe Perecin |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |