Autor(es)

Martins, Leticia S. ; Duarte, Evandro L. ; Lamy, M. Teresa ; Rozenfeld, Julio H. K.

• Tipo de documento: Artigo Científico
• Fonte: Biophysical Chemistry; v. 300, p. 8-pg., 2023-07-12.

Resumo

The saturated LPC18:0 and unsaturated LPC18:1 lysophosphatidylcholines have important roles in inflammation and immunity and are interesting targets for immunotherapy. The synthetic cationic lipid DODAB has been successfully employed in delivery systems, and would be a suitable carrier for those lysophosphatidylcholines. Here, assemblies of DODAB and LPC18:0 or LPC18:1 were characterized by Differential Scanning Calorimetry (DSC) and Electron Paramagnetic Resonance (EPR) spectroscopy. LPC18:0 increased the DODAB gel-fluid transition enthalpy and rigidified both phases. In contrast, LPC18:1 caused a decrease in the DODAB gel-fluid transition temperature and cooperativity, associated with two populations with distinct rigidities in the gel phase. In the fluid phase, LPC18:1 increased the surface order but, differently from LPC18:0, did not affect viscosity at the membrane core. The impact of the different acyl chains of LPC18:0 and 18:1 on structure and thermotropic behavior should be considered when developing applications using mixed DODAB membranes. (AU)

• Processo FAPESP: 16/19077-1 - Desenvolvimento de novos adjuvantes de vacinas capazes de ativar células T CD1d-restritas
• Beneficiário: Julio Henrique Kravcuks Rozenfeld
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/13368-4 - Sistemas nanoestruturados: de modelos biomiméticos de membranas a carreadores de bioativos
• Beneficiário: Karin Do Amaral Riske
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 21/01593-1 - Múltiplas técnicas físicas na caracterização estrutural de membranas lipídicas de interesse biológico
• Beneficiário: Maria Teresa Moura Lamy
• Modalidade de apoio: Auxílio à Pesquisa - Regular