Langmuir monolayers provide an effective strategy for studying molecular recognition of nucleobases using alkylated nucleotides

Autor(es)

Moreira, Felipe Almeida ; Escobar, Jhon Fernando Berrio ; Giordani, Cristiano ; Caseli, Luciano

• Tipo de documento: Artigo Científico
• Fonte: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 244, p. 8-pg., 2024-08-08.

Resumo

Molecular Recognition in nucleotides is crucial for medicine, underpinning precise interactions in genetic replication and therapy. Alkylated nucleotides, in particular, play a key role in modifying DNA to inhibit cancer cell growth. In this study, we focused on an alkylated nucleotide, PNM2 (3 ',4 ',6'-O-tristearoyl uridine or uridine tri-stearate), to investigate the interaction between adenine molecules in the aqueous subphase and PNM2 Langmuir monolayers. Utilizing techniques such as tensiometry, Brewster angle microscopy, infrared spectroscopy, surface potential measurements, and dilatational surface rheology, we found compelling evidence of molecular Recognition between the polar head of the insoluble amphiphile (uridine) in the monolayer and adenine in the aqueous subphase, attributed to hydrogen bonding. These interactions significantly influenced the physicochemical properties of the air-water interface, including monolayer expansion upon molecular recognition, decreased dilatational modulus, increased tensiometric stability of the monolayer when compressed to relevant surface pressures, and decreased surface potential. These findings are noteworthy for drug development, providing crucial insights into the mechanisms of nucleotide interactions. (AU)

• Processo FAPESP: 23/00850-6 - Desenvolvimento de biossensores nanoestruturados para diagnóstico de biomarcadores de desordens mentais
• Beneficiário: Osvaldo Novais de Oliveira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 22/03736-7 - Estudos sobre a interação de compostos bioativos em modelos de biointerface e construção de dispositivos nanoestruturados bioinspirados
• Beneficiário: Luciano Caseli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 22/13938-6 - Materiais nanoestruturados à base de nucleosídeos alquilados com propriedades anticancerígenos incorporados em filmes Langmuir e Langmuir-Blodgett de lipídeos e sondas luminescentes
• Beneficiário: Luciano Caseli
• Modalidade de apoio: Auxílio à Pesquisa - Regular