| Texto completo | |
| Autor(es): |
Ferronato, Giuliana de Avila
;
Vit, Franciele Flores
;
da Silveira, Juliano Coelho
Número total de Autores: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ANIMAL REPRODUCTION; v. 21, n. 1, p. 18-pg., 2024-01-01. |
| Resumo | |
In vitro cell culture is a well-established technique present in numerous laboratories in diverse areas. In reproduction, gametes, embryos, and reproductive tissues, such as the ovary and endometrium, can be cultured. These cultures are essential for embryo development studies, understanding signaling pathways, developing drugs for reproductive diseases, and in vitro embryo production (IVP). Although many culture systems are successful, they still have limitations to overcome. Three-dimensional (3D) culture systems can be close to physiological conditions, allowing greater interaction between cells and cells with the surrounding environment, maintenance of the cells' natural morphology, and expression of genes and proteins such as in vivo. Additionally, three-dimensional culture systems can stimulated extracellular matrix generating responses due to the mechanical force produced. Different techniques can be used to perform 3D culture systems, such as hydrogel matrix, hanging drop, low attachment surface, scaffold, levitation, liquid marble, and 3D printing. These systems demonstrate satisfactory results in follicle culture, allowing the culture from the pre-antral to antral phase, maintaining the follicular morphology, and increasing the development rates of embryos. Here, we review some of the different techniques of 3D culture systems and their applications to the culture of follicles and embryos, bringing new possibilities to the future of assisted reproduction. (AU) | |
| Processo FAPESP: | 21/06645-0 - Vesículas extracelulares como uma plataforma para diagnóstico e manipulação do sistema de produção in vitro de embriões: a nova geração da reprodução animal |
| Beneficiário: | Juliano Coelho da Silveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores - Fase 2 |
| Processo FAPESP: | 22/02701-5 - Criação de dispositivos microfluídicos com geometrias diferentes para fertilização in vitro |
| Beneficiário: | Franciele Flores Vit |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 19/25675-7 - Produção in vitro de embriões bovinos em diferentes sistemas de cultivo 3D: efeitos na morfologia nuclear, padrão de metilação, transcrição gênica e vesículas extracelulares |
| Beneficiário: | Giuliana de Avila Ferronato |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |