| Texto completo | |
| Autor(es): |
Arias, Cesar Andres Diaz
;
Matsudo, Marcelo Chuei
;
Ferreira-Camargo, Livia Seno
;
Molino, Joao Vitor Dutra
;
Mayfield, Stephen Patrick
;
de Carvalho, Joao Carlos Monteiro
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | CHEMICAL ENGINEERING & TECHNOLOGY; v. 47, n. 12, p. 9-pg., 2024-10-24. |
| Resumo | |
This study sought to evaluate the influence of five macronutrients (acetate, calcium, sulfate, nitrogen, and phosphate) on the cell growth and heterologous mCherry protein production by Chlamydomonas reinhardtii. In the first step, three nitrogen (N) sources were tested (NH4NO3, NaNO3, and NH4Cl), and NH4NO3 was selected as N source for the following step, due to the best results for mCherry protein production. In the second step, a central composite design 25 was employed to evaluate the effect of the five macronutrients. Although all nutrients had effect on maximum biomass concentration, only acetate, nitrogen, and phosphate showed to influence mCherry protein production. By employing the experimental design with small-scale culture in multiplates and multivariable regression, it was possible to achieve 12 % increase for recombinant protein production. (AU) | |
| Processo FAPESP: | 16/12992-6 - Chlamydomonas reinhardtii como plataforma de produção de biofármacos de interesse nacional |
| Beneficiário: | Lívia Seno Ferreira Camargo |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 13/18224-2 - Produção de proteína heteróloga em microalga |
| Beneficiário: | João Vitor Dutra Molino |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |