| Texto completo | |
| Autor(es): |
Harb, Samarah, V
;
Kolanthai, Elayaraja
;
Pinto, Leonardo A.
;
Beatrice, Cesar A. G.
;
Bezerra, Ewerton de O. T.
;
Backes, Eduardo H.
;
Costa, Lidiane C.
;
Seal, Sudipta
;
Pessan, Luiz A.
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Biomedical Materials; v. 19, n. 5, p. 16-pg., 2024-09-01. |
| Resumo | |
Bioactive and biodegradable scaffolds that mimic the natural extracellular matrix of bone serve as temporary structures to guide new bone tissue growth. In this study, 3D-printed scaffolds composed of poly (lactic acid) (PLA)-tricalcium phosphate (TCP) (90-10 wt.%) were modified with 1%, 5%, and 10 wt.% of ZnO to enhance bone tissue regeneration. A commercial chain extender named Joncryl was incorporated alongside ZnO to ensure the printability of the composites. Filaments were manufactured using a twin-screw extruder and subsequently used to print 3D scaffolds via fused filament fabrication (FFF). The scaffolds exhibited a homogeneous distribution of ZnO and TCP particles, a reproducible structure with 300 mu m pores, and mechanical properties suitable for bone tissue engineering, with an elastic modulus around 100 MPa. The addition of ZnO resulted in enhanced surface roughness on the scaffolds, particularly for ZnO microparticles, achieving values up to 241 nm. This rougher topography was responsible for enhancing protein adsorption on the scaffolds, with an increase of up to 85% compared to the PLA-TCP matrix. Biological analyses demonstrated that the presence of ZnO promotes mesenchymal stem cell (MSC) proliferation and differentiation into osteoblasts. Alkaline phosphatase (ALP) activity, an important indicator of early osteogenic differentiation, increased up to 29%. The PLA-TCP composite containing 5% ZnO microparticles exhibited an optimized degradation rate and enhanced bioactivity, indicating its promising potential for bone repair applications. (AU) | |
| Processo FAPESP: | 17/09609-9 - Desenvolvimento de scaffolds bioinspirados de PLA/cargas cerâmicas bioativas via impressão 3D |
| Beneficiário: | Luiz Antonio Pessan |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 19/27415-2 - Scaffolds bioativos com modificação superficial por plasma |
| Beneficiário: | Luiz Antonio Pessan |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 17/11366-7 - Desenvolvimento de scaffolds bioinspirados de PLA/biovidros via impressão 3D |
| Beneficiário: | Eduardo Henrique Backes |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 18/26060-3 - Scaffolds bioativos e bactericidas para regeneração óssea via impressão 3D |
| Beneficiário: | Samarah Vargas Harb |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |