Autor(es)

Duarte, Jonatas Lobato ; Di Filippo, Leonardo Delello ; Ribeiro, Tais de Cassia ; Silva, Ana Carolina de Jesus ; Hage-Melim, Lorane Izabel da Silva ; Duchon, Stephane ; Carrasco, David ; Pinto, Mara Cristina ; Corbel, Vincent ; Chorilli, Marlus

• Tipo de documento: Artigo Científico
• Fonte: PHARMACEUTICS; v. 16, n. 8, p. 14-pg., 2024-08-01.

Resumo

Aedes mosquito-borne diseases remain a significant global health threat, necessitating effective control strategies. This study introduces monoterpenes-based nanohydrogels for potential use as repellents against Aedes aegypti, the primary dengue vector worldwide. We formulated hydrogels using cymene- and myrcene-based nanoemulsions with different polymers: chitosan, carboxymethylcellulose (CMC), and carbopol (R). Our evaluations of rheological, texture, and bioadhesive properties identified CMC hydrogel as the most promising gelling agent for topical application, exhibiting sustained monoterpene release over 12 h with low skin permeation and high retention in the stratum corneum. Myrcene-loaded CMC hydrogel achieved a 57% feeding deterrence compared to 47% with cymene hydrogel in the mosquito membrane-feeding model. Molecular docking studies revealed interactions between myrcene and an essential amino acid (Ile116) in the Ae. aegypti odorant-binding protein 22 (AeOBP22), corroborating its higher repellent efficacy. These findings suggest that myrcene-loaded CMC hydrogels offer a promising, minimally invasive strategy for personal protection against Ae. aegypti and warrant further investigation to optimize monoterpene concentrations for vector control. (AU)

• Processo FAPESP: 21/11487-4 - Eficácia larvicida e repelente de nanoformulações contendo cimeno e mirceno contra Aedes aegypti L. resistentes e não resistentes
• Beneficiário: Jonatas Lobato Duarte
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 19/25125-7 - Nanoemulsões contendo terpenos como potenciais larvicidas e repelentes contra Aedes aegypti (Linnaeus, 1762)
• Beneficiário: Jonatas Lobato Duarte
• Modalidade de apoio: Bolsas no Brasil - Doutorado