| Texto completo | |
| Autor(es): |
Olean-Oliveira, Andre
;
Seraphim, Patricia Monteiro
;
Portugal, Miqueias L.
;
Teixeira, Marcos F. S.
Número total de Autores: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BIOSENSORS & BIOELECTRONICS; v. 275, p. 8-pg., 2025-05-01. |
| Resumo | |
Detection of glucose transporter 4 (GLUT4) is essential for understanding various physiological and pathological processes. This work reports the development of a novel electrochemical immunosensor for the direct detection of GLUT4, employing dissolved oxygen as a redox probe. This molecular oxygen-sensitive response is mediated by a redox-conductive polymer based on thionine. The sensor platform was fabricated via a one-step electropolymerization of thionine and gold nanoparticles (AuNPs) onto a platinum screen-printed electrode (OleanOliveira et al., 2022a). The immunosensor was then constructed by physical adsorption of a GLUT4 antibody onto the poly(thionine)-AuNP composite surface. This label-free approach eliminates the need for secondary antibodies or enzymes. The immunosensor performance was evaluated using electrochemical impedance spectroscopy (EIS). The sensing mechanism relies on impedance changes; increasing GLUT4 concentrations lead to increased impedance due to enhanced surface blocking upon GLUT4-antibody binding. This interaction impedes oxygen diffusion to the polymer redox sites, resulting in increased electrical resistance. Analysis of the redox capacitance as a function of frequency demonstrates a decrease in the capacitive arc with increasing GLUT4 concentration. (AU) | |
| Processo FAPESP: | 13/07296-2 - CDMF - Centro de Desenvolvimento de Materiais Funcionais |
| Beneficiário: | Elson Longo da Silva |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |
| Processo FAPESP: | 04/10130-0 - Avaliação da expressão de proteínas transportadoras de glicose (gluts) central e perifericamente em ratos resistentes à insulina |
| Beneficiário: | Patricia Monteiro Seraphim |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 24/08769-6 - DESENVOLVIMENTO DE UM GENOSSENSOR BASEADO EM NANOPARTICULA DE PRATA ENCAPSULADO EM POLÍMERO Pi-CONJUGADO PARA O MONITORAMENTO DE miRNA EM CELULAS PATOGENICAS |
| Beneficiário: | Miquéias de Lima Portugal |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |