Busca avançada
Ano de início
Entree


Resveratrol alters oral biofilm in vitro and in vivo

Texto completo
Autor(es):
Mostrar menos -
Reis, Fernanda Navas ; Camara, Joao Victor Frazao ; Abuna, Gabriel ; Moraes, Samanta Mascarenhas ; da Silva, Natara Dias Gomes ; Ventura, Talita Mendes Oliveira ; Araujo, Tamara Teodoro ; Rodrigues, Chelsea Maria Vilas Boas Feitosa ; Pardi, Vanessa ; Murata, Ramiro Mendonca ; Buzalaf, Marilia Afonso Rabelo
Número total de Autores: 11
Tipo de documento: Artigo Científico
Fonte: Journal of Dentistry; v. 152, p. 9-pg., 2024-11-26.
Resumo

Objective: To evaluate the ability of resveratrol to reduce dental caries in vitro and in vivo. Methods: In part 1, a microcosm biofilm protocol was employed. One hundred twenty-six bovine enamel specimens were treated with: Resveratrol (50, 100, 200, 400 mu g/mL), Phosphate buffered saline (negative control), Dimethyl sulfoxide (negative control) and 0.12% Chlorhexidine (positive control). The biofilm was produced from the saliva of 10 volunteers, under 0.2% sucrose exposure for 5 days, and daily treated with the solutions (1 min). At the end of the experimental period, resazurin and viable plate count assays were performed. Enamel demineralization was evaluated by transverse microrradiography (TMR). In part 2, 12 volunteers participated in a triple-blind crossover protocol for 7 weeks, according to the following treatments: 1) 100 mg/L resveratrol; 2) 0.05% NaF (226 mg/L F); 3) 100 mg/L resveratrol + 0.05% NaF; 4) Deionized water (negative control). Biofilm samples were collected from both sides of the mouth 12 h after the use of the solutions. Results: Resveratrol at 50 and 200 mu g/mL significantly reduced biofilm metabolic activity and mutans streptococci, respectively. Chlorhexidine was an effective treatment to significantly reduce all parameters, being an important antimicrobial and anticaries agent in vitro. Resveratrol alone or associated with NaF modulated several caries-associated bacteria in vivo. Conclusion: The present study represents the first step regarding the use of resveratrol within the concept of acquired enamel pellicle and biofilm engineering to prevent dental caries. (AU)

Processo FAPESP: 19/26070-1 - Modulação da película adquirida do esmalte e do biofilme para o controle da perda mineral dentária: desvendando mecanismos e possibilitando terapias
Beneficiário:Marília Afonso Rabelo Buzalaf
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 22/07905-8 - Resveratrol para a prevenção da cárie dentária: um estudo in vivo com análise do microbioma oral
Beneficiário:Fernanda Navas Reis
Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Mestrado
Processo FAPESP: 20/13351-0 - Resveratrol para prevenção da cárie e erosão dentária: estudos in vitro
Beneficiário:Fernanda Navas Reis
Modalidade de apoio: Bolsas no Brasil - Mestrado