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Magnani, Rodrigo Facchini ; Volpe, Haroldo Xavier Linhares ; Luvizotto, Rejane Angelica Grigio ; Mulinari, Tatiana Aparecida ; Agostini, Thiago Trevisoli ; Bastos, Jairo Kenupp ; Ribeiro, Victor Pena ; Carmo-Sousa, Michele ; Wulff, Nelson Arno ; Pena, Leandro ; Leal, Walter S.

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 15, n. 1, p. 10-pg., 2025-01-28.

Resumo

The Asian Citrus Psyllid (ACP), Diaphorina citri, severely threatens citrus production worldwide by transmitting the greening (= Huanglongbing)-causing bacterium Candidatus Liberibacter asiaticus. There is growing evidence that the push-pull strategy is suitable to partially mitigate HLB by repelling ACP with transgenic plants engineered to produce repellents and attracting the vector to plants with a minimal disease transmission rate. Species that pull ACP away from commercial citrus plants have been identified, and transgenic plants that repel ACP have been developed. The concept of a repellent-producing plant was first demonstrated with an Arabidopsis line engineered to overexpress a gene controlling the synthesis of beta-caryophyllene and other sesquiterpenes. We have analyzed the volatile organic compounds released by this Arabidopsis line and identified alpha-humulene, alpha-copaene, and trace amounts of beta-elemene, in addition to beta-caryophyllene. Behavioral measurements demonstrated that alpha-copaene repels ACP at doses ca. 100x lower than those needed for beta-caryophyllene repellence. In contrast, alpha-humulene is innocuous at the level emitted by the transgenic plant. We confirmed that a mixture of the three sesquiterpenes in the ratio 1:100:10 repels ACP. Likewise, a commercial sample of copaiba oil containing the three sesquiterpenes, in a proportion similar to that in the transgenic plant, repelled ACP. (AU)

• Processo FAPESP: 17/21460-0 - Medidas para a redução das infecções primárias no manejo integrado de Huanglongbing no cinturão citrícola de São Paulo: Viabilidade técnica e econômica
• Beneficiário: Renato Beozzo Bassanezi
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 19/13799-3 - Emu concedido no processo 2017/21460-0: cromatógrafo gasoso acoplado a um detector de espetrometria de massas triploquadrupolar sequencial (gc-ms/ms)
• Beneficiário: Renato Beozzo Bassanezi
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 15/07011-3 - Estratégias biotecnológicas para o controle do hlb mediante transgenia
• Beneficiário: Leandro Antonio Peña Garcia
• Modalidade de apoio: Auxílio à Pesquisa - Temático