| Texto completo | |
| Autor(es): |
Rodolpho, B. T.
;
Bertagna, N. B.
;
Favoretto, C. A.
;
Moretti, N. S.
;
Cruz, F. C.
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Physiology & Behavior; v. 291, p. 7-pg., 2025-03-15. |
| Resumo | |
Alcohol use disorder (AUD) is a condition with multifactorial causes, including biopsychosocial factors. Childhood exposure to stress may increase susceptibility to AUD in adulthood. Despite its significance, the interaction between stress and AUD remains unclear. This study investigated whether maternal separation (MS) stress would change epigenetic marker levels in mice's amygdala and whether these changes associate with increased ethanol consumption and preference in adulthood. C57BL/6J pups in the MS group were removed from their nests for 3 h daily from postnatal day (PND) 1 to PND 14. Control animals remained under maternal care. All litters were weaned on PND 21, and on PND 60, mice were subjected to a two-bottle choice protocol using one bottle containing water and another containing ethanol in crescent concentrations (5 %, 10 %, 15 %, and 20 % w/v; every three days) for 8 h daily. Following a 3-day withdrawal, a reinstatement test using the two-bottle choice paradigm was conducted. Afterward, the amygdala was dissected for analysis of acetylated histones, H3 dimethylated in lysine 9, Sirtuin-1, and DNA methyltransferases-1 by Western Blotting. Results demonstrated that exposure to MS during childhood increased mice ethanol preference but not consumption during adulthood. We also observed no alterations in the levels of the epigenetic markers analyzed. These results support the hypothesis that MS exposure can influence ethanol-related behaviors in the later phases of development. (AU) | |
| Processo FAPESP: | 20/04389-3 - Influência do estresse de separação materna sobre os comportamentos relacionados à dependência de etanol em camundongos: análise da neurotransmissão CRFérgica |
| Beneficiário: | Natalia Bonetti Bertagna |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 20/01331-4 - Efeito das alterações epigenéticas no sistema de nociceptina promovidas pelo estresse de separação materna sobre o consumo de etanol em camundongos. |
| Beneficiário: | Ben Tagami Rodolpho |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 19/24073-3 - Papel dos receptores canabinóides CB1 na modulação de circuitos neurais envolvidos na interação entre o estresse de separação materna e consumo de etanol em camundongos |
| Beneficiário: | Cristiane Aparecida Favoretto |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 22/03075-0 - Desvendando os mecanismos regulatórios envolvidos na relação parasito hospedeiro da Leishmania: foco nas alterações pós-transcricionais e pós-traducionais |
| Beneficiário: | Nilmar Silvio Moretti |
| Modalidade de apoio: | Auxílio à Pesquisa - Projeto Inicial |