| Texto completo | |
| Autor(es): |
Pessoa, Jassiara da Silva
;
da Silva, Bruno Guzzo
;
de Franca Jr, Ednilson Donisete
;
da Silva Filho, Isac Jose
;
Molino, Joao Vitor Dutra
;
de Carvalho, Joao Carlos Monteiro
;
Ferreira-Camargo, Livia Seno
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF BASIC MICROBIOLOGY; v. N/A, p. 8-pg., 2025-02-12. |
| Resumo | |
Chlamydomonas reinhardtii is a promising model microalga for recombinant molecules production. Nonetheless, low yield is a challenge for its industrial use. This work investigated the influence of ammonium chloride (NH4Cl) concentration and temperature on the growth of transgenic C. reinhardtii expressing the fluorescent protein mCherry on a laboratory scale. A Central Composite Rotatable Design was used to establish the cultivation conditions. NH4Cl concentrations ranging from 400 to 647.49 mg/L and temperatures between 25 degrees C and 32.1 degrees C resulted in maximum values of cell concentration and mCherry fluorescence. Lower temperatures (15 degrees C-17 degrees C) were found to be more suitable for the accumulation of total soluble proteins. These results demonstrate that cultivation conditions can positively affect C. reinhardtii growth, with a range of conditions that can be used. Unlike genetic approaches, this study provides a solution to enhance both growth and recombinant protein expression in C. reinhardtii. These findings pave the way for scaling up the use of C. reinhardtii as a biofactory in industry and can be applied to other microalgal systems. (AU) | |
| Processo FAPESP: | 16/12992-6 - Chlamydomonas reinhardtii como plataforma de produção de biofármacos de interesse nacional |
| Beneficiário: | Lívia Seno Ferreira Camargo |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 19/13822-5 - Acúmulo da proteína recombinante terapêutica somatotropina em Chlamydomonas reinhardtii |
| Beneficiário: | Jassiara da Silva Pessoa |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |