Determination of sildenafil in pharmaceutical formulations and synthetic urine using a composite electrode composed of acetylene black modified with silver nanoparticles and vegetable oil-derived polyurethane

Autor(es)

da Silva, Rafael ; Alarcon, Rafael Turra ; Cavalheiro, Eder Tadeu Gomes

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ELECTROANALYTICAL CHEMISTRY; v. 979, p. 12-pg., 2025-02-15.

Resumo

The development of electrode materials as alternatives to mercury and noble metals became an important branch of research in electroanalytical chemistry. Sildenafil is a widely marketed vasodilator drug used to treat erectile dysfunction. In this work, an acetylene black (AB)-based composite electrode with silver nanoparticles deposited on the surface and a vegetable oil polyurethane is proposed as a new voltammetric sensor. The deposition of silver on the AB surface was selected from different procedures. Thermal reduction in the presence of ethylene glycol resulted in higher yield, according to thermogravimetry and energy-dispersive X-ray spectroscopy. The modified AB particles were used as the conducting phase in composite electrodes agglutinated by polyurethane and the resulting devices after optimizing the experimental and instrumental conditions. The electrode was then used in the determination of sildenafil in pharmaceutical formulations and synthetic urine in 0.10 mol/L phosphate buffer, pH 3.0, resulting in a limit of detection of 0.89 mu mol/L. The results were in agreement with both the label values and the amount of drug spiked in the synthetic urine. The analyses of the pharmaceutical samples agreed within the 95 % confidence level, according to the Student's t-test, when compared to the chromatographic determination. (AU)

• Processo FAPESP: 19/22217-8 - Estudos termoanalíticos aplicados a fármacos antivirais antihepatite e antiretrovirais da classe dos inibidores de protease: comportamento térmico, intermediários de decomposição, caracterização de voláteis e polimorfismo
• Beneficiário: Éder Tadeu Gomes Cavalheiro
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 22/15211-6 - Preparação e Aplicação de Eletrodos Compósitos à Base de Grafite e Polímeros: miniaturização, eletrodos impressos e sensores flexíveis
• Beneficiário: Éder Tadeu Gomes Cavalheiro
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 21/14879-0 - Novo método para síntese de polihidróxiuretanos a partir de triacilglicerol e utilizando catalisadores ou triacilmelamina, visando inibir reações paralelas
• Beneficiário: Rafael Turra Alarcon
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado