Autor(es)

Bello, Vinicius Henrique ; Pires, Lorenna Leal ; Pires, Gleice Rafaela Renunza ; Bernardi, Gustavo Verruma ; Ramos-Gonzalez, Pedro Luis ; Salaroli, Renato Barbosa ; Kitajima, Elliot Watanabe

• Tipo de documento: Artigo Científico
• Fonte: CIENTIA AGRICOL; v. 82, p. 11-pg., 2025-01-01.

Resumo

An isolate of the tobamovirus tobacco mild green mosaic virus (TMGMV), Tobamovirus mititessellati, was found naturally infecting petunia (Petunia x hybrida) cv. Pinstripe, causing reduced leaf size, mottling, and color breaking of flowers, in an experimental field at Piracicaba, S & atilde;o Paulo state, Brazil. TMGMV was identified by virion morphology, cytopathology, transmission experiments, and molecular assays. The entire nucleotide sequence of this petunia isolate of TMGMV (TMGMV-Pet) was obtained and demonstrated, in essence, to be similar to previously analyzed isolates. One hundred and twenty plant species were assayed by mechanical transmission, and 35 of them from nine families, both previously reported and unreported species, were shown to be experimentally susceptible to TMGMV-Pet. On the other hand, certain species, among 85 unsusceptible hosts for TMGMV-Pet, known to be susceptible to TMGMV, failed to be infected. Cytopathological studies on naturally and experimentally infected host plants revealed that virions of TMGMV-Pet produced para-crystalline aggregates, but not angular arrays, which are considered characteristic of TMGMV. As previously described, thin sections revealed the presence of pseudo-virions in the stroma of chloroplasts in TMGMV-Pet-infected tobacco (Nicotiana tabacum), and, additionally, in other hosts such as N. clevelandii, N. megalosyphon, and petunia indicating that this process is not restricted to tobacco. (AU)

• Processo FAPESP: 21/02179-4 - Avaliação da diversidade dos patógenos moleculares (vírus e viroides de plantas) no Brasil: Fase III - 2021/2023
• Beneficiário: Elliot Watanabe Kitajima
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular
• Processo FAPESP: 20/05563-7 - A hipótese do amplifier host na epidemiologia do Mosaico Dourado do tomateiro, causado pelo Tomato Severe Rugose Virus (ToSRV): comprovação experimental e determinação das condições de ocorrência
• Beneficiário: Vinicius Henrique Bello
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 22/05043-9 - Solano-violeta como hospedeira natural de três vírus distintos transmitidos por ácaros Brevipalpus: modelo para estudos de interação entre estes vírus
• Beneficiário: Gustavo Verruma Bernardi
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica