| Texto completo | |
| Autor(es): |
Souza, Alann Thaffarell Portilho
;
Freitas, Gileade Pereira
;
Lopes, Helena Bacha
;
Weffort, Denise
;
Adolpho, Leticia Faustino
;
Gomes, Maria Paula Oliveira
;
Oliveira, Fabiola Singaretti
;
Almeida, Adriana Luisa Gonsalves
;
Beloti, Marcio Mateus
;
Rosa, Adalberto Luiz
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ARCHIVES OF MEDICAL RESEARCH; v. 56, n. 6, p. 12-pg., 2025-09-01. |
| Resumo | |
Background. Diabetes mellitus (DM) negatively impacts bone tissue, leading to bone loss and increased fracture risk. Many individuals need additional treatments, and therapy based on mesenchymal stromal cells (MSCs) represents a promising treatment for bone defects in patients with diabetes. Aims. The present study explored the effects of interactions between MSCs from normoglycemic (NG-MSCs) and diabetic (DM-MSCs) donors on osteoblast differentiation and the effects of cell therapy using NG-MSCs on bone regeneration in defects created in diabetic rats. Methods. After inducing DM with streptozotocin, we evaluated the morphometric parameters of rat femurs and the osteoblast differentiation of MSCs, as well as the effects of the interaction between NG-MSCs and DM-MSCs on their osteoblast differentiation. The efficacy of cell therapy was measured by evaluating the bone repair in calvarial defects of diabetic rats treated with local injections of either NG-MSCs or a vehicle. Results. DM induced bone loss and impaired the osteoblast differentiation of MSCs, which was partially restored by NG-MSCs, while the bone formation observed in defects treated with NG-MSCs and the vehicle was similar. Conclusion. These results indicate that the beneficial effect of NG-MSCs on DM-MSCs did not translate into enhanced bone repair, mainly due to a hostile environment created by hyperglycemia, which compromised the ability of MSCs to induce bone formation. (c) 2025 Instituto Mexicano del Seguro Social (IMSS). Published by Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies. (AU) | |
| Processo FAPESP: | 19/08048-9 - Efeito da mutação de Ggps1 nas funções de células ósseas in vitro e na fragilidade diafisária in vivo usando camundongos após cirurgia de ovariectomia e expostos ao tratamento de longa duração com bifosfonatos contendo nitrogênio |
| Beneficiário: | Alann Thaffarell Portilho de Souza |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado |
| Processo FAPESP: | 18/13290-0 - Terapia celular: avaliação do efeito de células-tronco mesenquimais para regenerar tecido ósseo de ratos com Osteoporose, Diabetes Mellitus ou Hipertensão Arterial |
| Beneficiário: | Alann Thaffarell Portilho de Souza |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 17/12622-7 - Terapia celular: potencial de células-tronco mesenquimais, VEGF-A e BMP-9 para regenerar tecido ósseo |
| Beneficiário: | Adalberto Luiz Rosa |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |