| Texto completo | |
| Autor(es): Mostrar menos - |
Ninni, Andrea
;
Zaccaria, Fabio
;
Verteramo, Luca
;
Sciarretta, Francesca
;
Silveira, Loreana Sanches
;
Rosa-Neto, Jose Cesar
;
Carotti, Simone
;
Nevi, Lorenzo
;
Grumati, Paolo
;
Patel, Satish
;
Carrera, Giulia
;
Sgambato, Alessandro
;
Lucchetti, Donatella
;
Colella, Filomena
;
Severi, Ilenia
;
Senzacqua, Martina
;
Giordano, Antonio
;
Bernardini, Sergio
;
Di Biagio, Claudia
;
Tortolici, Flavia
;
Rizzo, Giuseppe
;
Cochain, Clement
;
Chiurchiu, Valerio
;
Ivanov, Stoyan
;
Zhou, Beiyan
;
Williams, Jesse W.
;
Savage, David B.
;
Aquilano, Katia
;
Lettieri-Barbato, Daniele
Número total de Autores: 29
|
| Tipo de documento: | Artigo Científico |
| Fonte: | NATURE COMMUNICATIONS; v. 16, n. 1, p. 16-pg., 2025-05-31. |
| Resumo | |
Macrophages are key regulators of adipose tissue plasticity. Obesity impairs brown adipose tissue (BAT) function in humans, yet macrophage-mediated mechanisms remain elusive. Here, we introduce MACanalyzeR, a single-cell RNA sequencing (scRNAseq) tool designed for comprehensive monocyte/macrophage metabolic profiling. Applying MACanalyzeR to BAT from obese male murine models (db/db and HFD-fed mice), we identify lipid-associated macrophages (LAMs) with foamy characteristics. Unlike db/db BAT LAMs, those in HFD BAT correlate with thermogenic gene expression and PPAR signaling activation. A distinct PpargHIGH LAM subcluster progressively accumulates in thermogenically active BAT. Macrophage-specific Pparg depletion disrupts BAT thermogenesis, inducing a white-like phenotype and metabolic dysfunctions. Mechanistically, PpargHIGH LAMs secrete GDF15, a key regulator of BAT identity and lipid metabolism under high-energy demand. Our study establishes MACanalyzeR as a powerful tool for immunometabolic interrogation and identifies PpargHIGH LAMs as critical mediators of BAT homeostasis. (AU) | |
| Processo FAPESP: | 19/09679-2 - Caracterização da subpopulação de macrófagos com deleção de PPARgamma em modelo de câncer de mama e a influência do treinamento aeróbio moderado |
| Beneficiário: | Loreana Sanches Silveira |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 22/06073-9 - Efeito dos clock genes sobre a regulação do imunometabolismo e diferenciação de monócitos/macrófagos durante a imunossenescência: o papel do treinamento físico ao longo da vida |
| Beneficiário: | José Cesar Rosa Neto |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |