Rho Signaling Pathway and Apical Constriction in the Early Lens Placode

Autor(es)

Borges, Ricardo Moraes ^[1] ; Lamers, Marcelo Lazzaron ^[2] ; Forti, Fabio Luis ^[3] ; dos Santos, Marinilce Fagundes ^[1] ; Irene Yan, Chao Yun ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Biol Celular & Desenvolvimento, BR-05508000 Sao Paulo - Brazil

^[2] Univ Fed Rio Grande do Sul, Dept Ciencias Morfol, Inst Ciencias Basicas Saude, BR-90046900 Porto Alegre, RS - Brazil

^[3] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-01498 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: GENESIS; v. 49, n. 5, p. 368-379, MAY 2011.
• Citações Web of Science: 20

Resumo

Epithelial invagination in many model systems is driven by apical cell constriction, mediated by actin and myosin II contraction regulated by GTPase activity. Here we investigate apical constriction during chick lens placode invagination. Inhibition of actin polymerization and myosin II activity by cytochalasin D or blebbistatin prevents lens invagination. To further verify if lens placode invaginate through apical constriction, we analyzed the role of Rho-ROCK pathway. Rho GTPases expression at the apical portion of the lens placode occurs with the same dynamics as that of the cytoskeleton. Overexpression of the pan-Rho inhibitor C3 exotoxin abolished invagination and had a strong effect on apical myosin II enrichment and a mild effect on apical actin localization. In contrast, pharmacological inhibition of ROCK activity interfered significantly with apical enrichment of both actin and myosin. These results suggest that apical constriction in lens invagination involves ROCK but apical concentration of actin and myosin are regulated through different pathways upstream of ROCK. genesis 49: 368-379, 2011. (C) 2011 Wiley-Liss, Inc. (AU)

• Processo FAPESP: 07/57450-7 - Mecanismos celulares na morfogênese do placódio do cristalino
• Beneficiário: Chao Yun Irene Yan
• Modalidade de apoio: Auxílio à Pesquisa - Regular