Autor(es)

Garcia-Fossa, Fernanda ; Moraes-Lacerda, Thais ; Rodrigues-da-Silva, Mariana ; Diaz-Rohrer, Barbara ; Singh, Shantanu ; Carpenter, Anne E. ; Cimini, Beth A. ; de Jesus, Marcelo Bispo

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR BIOLOGY OF THE CELL; v. 36, n. 7, p. 11-pg., 2025-07-01.

Resumo

Image-based profiling has been used to analyze cell health, drug mechanism of action, CRISPR-edited cells, and overall cytotoxicity. Cell Painting is a broadly used imagebased assay that uses morphological features to capture how cells respond to treatments. However, this method requires cell fixation for staining, which prevents examining live cells. To address this limitation, here we present Live-Cell Painting (LCP), a high-content method based on acridine orange, a metachromatic dye that labels different organelles and cellular structures. We began by showing that LCP can be applied to follow acidic vesicle redistribution of cells exposed to acidic vesicles inhibitors. Next, we show that LCP can identify subtle changes in cells exposed to silver nanoparticles that are not detected by techniques such as MTT assay. In drug treatments, LCP was helpful in assessing the dose-response relationship and creating profiles that allow clustering of drugs that cause liver injury. Here, we present an affordable and easy-to-use image-based assay capable of assessing overall cell health and showing promise for use in various applications such as assessing drugs and nanoparticles. We envisage the use of LCP as an initial screening of overall cell health while providing insights into new biological questions. (AU)

• Processo FAPESP: 19/24033-1 - Alterações sutis desencadeadas por nanopartículas em células analisadas individualmente através de perfil multiparamétrico baseada em imagem
• Beneficiário: Fernanda Garcia Fóssa
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 23/06143-0 - Aspectos moleculares da interação nanopartículas e células: atividade terapêutica, entrega de oligonucleotídeos e nanotoxicidade
• Beneficiário: Eneida de Paula
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 22/01483-4 - Alterações sutis desencadeadas por nanopartículas em células analisadas individualmente através de perfil multiparamétrico baseada em imagem
• Beneficiário: Fernanda Garcia Fóssa
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 20/01218-3 - Nanotoxicidade analisada individualmente através de perfil multiparamétrico baseada em imagem
• Beneficiário: Marcelo Bispo de Jesus
• Modalidade de apoio: Auxílio à Pesquisa - Regular