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Social Isolation Induces Sex-Differentiated Sensory-Emotional Pain Responses and Oxytocinergic Receptors Alterations in Mice

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Autor(es):
Braga-Dias, Ana Claudia ; Cardenas Otero, Silvia Jimena ; Cavalcante Oliveira, Melissa Kessia ; Canto-de-Souza, Lucas ; Crestani, Carlos C. ; Canto-de-Souza, Azair ; Baptista-de-Souza, Daniela ; Nunes-de-Souza, Ricardo Luiz
Número total de Autores: 8
Tipo de documento: Artigo Científico
Fonte: EUROPEAN JOURNAL OF PAIN; v. 29, n. 8, p. 15-pg., 2025-09-01.
Resumo

Background Chronic pain is a global health problem frequently associated with psychopathologies such as anxiety and depression, with a higher prevalence in women. However, most of the preclinical studies have used male animals as experimental subjects, highlighting the importance of studies that consider sex as a biological factor modulating the responses related to chronic pain. The experience of high social stress can maintain and exacerbate chronic pain. From a neural perspective, oxytocin plays a critical role in modulating behavioural and neurobiological responses related to stress and pain.Methods Therefore, this study aimed to evaluate the sensory and emotional effects of social isolation in male and female mice subjected to chronic constriction injury (CCI). Additionally, it aims to investigate the role of oxytocinergic neurotransmission in chronic pain across different sexes.Results The results demonstrated that both sciatic nerve constriction (CCI) and social isolation independently induced mechanical hypersensitivity and increased anxiety- and depression-like responses in both male and female mice. Despite no synergistic interaction, social isolation independently triggered sensory and emotional changes comparable to those induced by CCI. However, only females exhibited an increase in facial pain expressions and a worsening of pelage condition when housed individually. Moreover, females, but not males, had a decreased expression of oxytocin receptors in the hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei under social isolation.Conclusions These findings indicate that social isolation can induce sexually dimorphic behavioural and neurophysiological responses in mice.Significance Statement This article shows that social isolation enhances pain perception and oxytocinergic neurotransmission, especially in female mice. This understanding of how pain affects different sexes may contribute to developing a sex-specific new therapeutic target to assist individuals living with pain. (AU)

Processo FAPESP: 22/06260-3 - Avaliação de lateralização funcional no controle das respostas cardiovasculares e comportamental ao medo condicionado ao contexto pelo córtex pré-frontal medial em ratos
Beneficiário:Carlos Cesar Crestani
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 22/12932-4 - Influência das diferenças sexuais e da neurotransmissão ocitocinérgica nos componentes sensitivos e afetivos da dor crônica em camundongos submetidos ao isolamento social
Beneficiário:Ana Claudia Braga Dias
Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 23/13491-4 - Impacto do dimorfismo sexual e da neurotransmissão ocitocinérgica sobre a dor crônica em modelos animais e em humanos
Beneficiário:Daniela Baptista de Souza
Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores
Processo FAPESP: 22/04387-6 - Impacto do dimorfismo sexual e da neurotransmissão ocitocinérgica sobre a dor crônica em modelos animais e em humanos
Beneficiário:Daniela Baptista de Souza
Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores