| Texto completo | |
| Autor(es): |
Altafini, Rafael de Moraes
;
Fachin, Giovana Masson
;
Reginatto, Valeria
Número total de Autores: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | FERMENTATION-BASEL; v. 11, n. 7, p. 15-pg., 2025-06-26. |
| Resumo | |
Biodiesel is one of the most important biofuels worldwide. Besides glycerol, the residual aqueous phase of the transesterification reaction (RAPTR) from the biodiesel industry contains a high concentration of methanol. Here, we propose using RAPTR as substrate for Clostridium beijerinckii Br21 to produce 1,3-propanediol (1,3-PDO). 1,3-PDO is a valuable chemical compound widely used in the production of polymers, cosmetics, and pharmaceuticals. To diminish the methanol content, we pretreated RAPTR by low-pressure evaporation, which minimized water evaporation and prevented other contaminants from being concentrated. We optimized the evaporation conditions by using a 22 central composite rotational design to establish optimal temperature and time of 55 degrees C and 51.3 min, respectively. Pretreated RAPTR diluted at 20% (v v-1) with a nutrient solution allowed the bacterium to grow, but no glycerol was consumed. Supplementing the nutrient solution with 0.4 g L-1 MgCl2, defined in another experimental design, led the bacterium to consume glycerol and to produce 1,3-PDO. In the optimized conditions, pretreated RAPTR supplemented with MgCl2 gave 2.78 +/- 0.01 g L-1 1,3-PDO in higher yield (Y1,3-PDO/glycerol) compared to the theoretical one, 0.61 and 0.50 g g-1, respectively. This result is relevant for biodiesel biorefineries, which could implement the innovative and customized strategy proposed herein to obtain 1,3-PDO, a high-value-added product, from a glycerol- and methanol-rich residue. (AU) | |
| Processo FAPESP: | 22/04024-0 - Cofermentação de resíduos orgânicos e inorgânicos em sistemas biológicos e bioeletroquímicos para a obtenção de bioprodutos e eletricidade |
| Beneficiário: | Valeria Reginatto Spiller |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Regular |