Autor(es)

Martorano, Antonio Secco ; Reggio, Camilla ; de Almeida, Luciana Oliveira ; Scolaro, Cristina ; de Castro-Raucci, Larissa Moreira Spinola ; Spriano, Silvia ; de Oliveira, Paulo Tambasco

• Tipo de documento: Artigo Científico
• Fonte: APPLIED SURFACE SCIENCE ADVANCES; v. 29, p. 15-pg., 2025-09-01.

Resumo

The aim of this study was to evaluate fibrinogen (FG) coating on a nanostructured titanium surface (Ti) regarding its physicochemical properties and the response of pre-osteoblastic cells in vitro. Commercially pure Ti discs were ground and chemically treated with a 1:1 mixture of 30% hydrogen peroxide and concentrated sulfuric acid (Nano-Ti). FG coating was performed by simple adsorption at its plasma concentration (FG/Nano-Ti). Preosteoblastic MC3T3-E1 cells were plated on Nano-Ti and FG/Nano-Ti and cultured for up to 18 days. FG/ Nano-Ti resulted in the availability of 0.7 mg/cm2 of protein on FG/Nano-Ti (BCA). Nano-Ti and FG/Nano-Ti exhibited microscale grooves and a typical nano-topography, with a network of nanopores, which was partially masked for FG/Nano-Ti by the presence of granulated material of homogeneous distribution. The adsorbed protein layer was continuous and probably a few nanometers thick, not affecting the micro-grooves of Nano-Ti. The roughness parameters exhibited higher values for FG/Nano-Ti. Zeta potential titration curves showed an isoelectric point at pH 3.5 for Nano-Ti evidencing hydroxyl functional groups with a weak acidic reactivity and 5.5 for FG/Nano-Ti, which is the same as FG, in agreement with a continuous adsorbed layer. The presence of FG reduced the wettability of the nano-topography in contact with a drop of water or fetal bovine serum, but enhanced it when a blood drop was used. The biological results showed a higher expression of classical osteoblast markers - especially RUNX2 - on FG/Nano-Ti, which corresponded to higher values of alkaline phosphatase activity and mineralization of the cultures. When FG/Nano-Ti was exposed to exogenous thrombin, a homogeneous fibrin fibril was assembled. In conclusion, the strategy of coating FG/Nano-Ti with FG potentiates the capacity of Nano-Ti to promote osteogenic differentiation. (AU)

• Processo FAPESP: 16/50298-4 - Funcionalização de superfície de materiais bioativos com moléculas naturais para a modulação da resposta tecidual a implantes aloplásticos
• Beneficiário: Paulo Tambasco de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular