Autor(es)

Silveira, Natalia Nascimento ; Andrada, Heber Eduardo ; Paulino, Julia Mirian ; Boaretto, Naiara Cristina da Silva ; Molina, Eduardo Ferreira ; dos Santos, Raquel Alves

• Tipo de documento: Artigo Científico
• Fonte: FUTURE PHARMACOLOGY; v. 5, n. 3, p. 22-pg., 2025-09-18.

Resumo

Backgorund/Objectives: Advances in nanotechnology have enabled conventional compounds with low bioavailability to achieve their full therapeutic potential by ensuring targeted tissue delivery. In this context, polymeric nanogels have emerged as a promising option for drug delivery due to their high loading capacity and excellent in vivo stability. Objectives: Given the growing potential of nanogels in drug delivery, their cytotoxicity and genotoxicity must be evaluated to ensure safety in biotechnological applications. This study assessed the genotoxic safety of nanogels synthesized via the reaction of Jeffamine (R) T-5000 polyoxypropylene triamine (PPO) monomers (Huntsman Chemical, The Woodlands, TX, USA) and poly (ethylene glycol) diglycidyl ether (DPEG) in varying proportions: 1:1 (Nano11), 1:3 (Nano13), and 2:3 (Nano23) PPO/DPEG. Additionally, we determined which of the two components exhibited lower toxicity against the CHO-K1 cell line (Chinese hamster ovary). Methods: To achieve this, short- and long-term cytotoxicity experiments were conducted using the XTT colorimetric assay and clonogenic survival assay, alongside the micronucleus test and comet assay for genotoxicity analysis. Results: The cytotoxicity assays (XTT, clonogenic, and trypan blue) indicated that the nanogels did not exhibit cytotoxic effects at concentrations up to 100 mu g/mL, while the genotoxicity assays revealed no evidence of DNA or chromosomal damage at these levels. Conclusions: These findings underscore the safety profile of Jeffamine (R) T-5000 as an effective carrier, demonstrating its compatibility with DPEG and positioning it as a highly promising and innovative solution for advanced drug delivery systems. (AU)

• Processo FAPESP: 21/14619-9 - EMU concedido no processo 2021/06552-1: equipamento DLS/potencial zeta
• Beneficiário: Eduardo Ferreira Molina
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 21/06552-1 - TASTYGEL: liberação sustentável de nutrientes através de inteligentes micro- e nanogéis baseados em polieteraminas-bisepóxido para aplicações na Agricultura
• Beneficiário: Eduardo Ferreira Molina
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores - Fase 2
• Processo FAPESP: 22/06507-9 - Liberação simultânea de micronutrientes a partir de nanogéis à base de polieteraminas e bisepóxido
• Beneficiário: Heber Eduardo Andrada
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado