Autor(es)

Dias, Marina Ceccon ; Silva, Caio Cesar Gonsalves ; Rojas-Mantilla, Hernan Dario ; Silva, Lucas Moreira ; Garrido, Saulo Santesso ; Zanoni, Maria Valnice Boldrin

• Tipo de documento: Artigo Científico
• Fonte: Microchemical Journal; v. 218, p. 10-pg., 2025-09-18.

Resumo

Photobioelectrochemical systems have gained prominence in environmental contaminant monitoring due to their high sensitivity, selectivity, and ability to operate under visible light. In this study, we investigated the incorporation of chloroplasts, the organelle responsible for photosynthesis, into a tungsten trioxide (WO3) n-type semiconductor electrode modified with a thin polydopamine (PDA) layer. PDA plays a crucial role in efficiently immobilizing chloroplasts, facilitating electron transfer, and enhancing light energy conversion. The biosensor's performance was evaluated based on its photocurrent response in the presence and absence of simazine (SMZ), a widely used herbicide classified as an emerging pollutant. The proposed methodology exhibited a linear response in the range of 0.005 to 10.0 mu M, with a detection limit of 0.002 mu M (0.410 ppb) and a quantification limit of 0.004 mu M (0.759 ppb). Its application in aqueous samples (tap, mineral, river, groundwater) demonstrated good recovery rates. The electrode efficiency response was compared with chromatographic analysis (LC/MS/MS and GC/MS), which provided similar results, confirming the accuracy and reliability of the device. Additionally, the sustainability of the method was assessed using AGREE software, where the biosensor achieved a score of 0.78, indicating a significantly greener profile compared to the chromatograph (score of 0.55). This study establishes the chloroplast-based biophotoelectrode as a viable, sustainable, and cost-effective alternative for SMZ herbicide monitoring in environmental samples, combining analytical efficiency with reduced environmental impact. (AU)

• Processo FAPESP: 14/50945-4 - INCT 2014: Instituto Nacional de Tecnologias Alternativas para Detecção, Avaliação Toxicológica e Remoção de Micropoluentes e Radioativos
• Beneficiário: Maria Valnice Boldrin
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 23/17973-3 - Desenvolvimento de biossensor impedimétrico baseado em eletrodo descartável impresso em papel (SPE) para diagnóstico precoce da ferrugem do cafeeiro
• Beneficiário: Lucas Moreira Silva
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 24/04167-1 - Membranas de filtração modificadas com semicondutores para construir sistemas híbridos filtração/fotocatálise heterogênea.
• Beneficiário: Hernan Dario Rojas Mantilla
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado