| Texto completo | |
| Autor(es): |
Vespasiano, Caroline Tie
;
Serino-Silva, Caroline
;
Silveira, Giovanni Perez Machado
;
Rodrigues, Fabiola Souza
;
Rameh-de-Albuquerque, Luciana Carla
;
Sant'anna, Savio Stefanini
;
Grego, Kathleen Fernandes
;
Tanaka-Azevedo, Anita Mitico
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Toxicon; v. 267, p. 11-pg., 2025-11-01. |
| Resumo | |
Snake envenoming is a relevant public health issue, especially in tropical regions worldwide. Bothrops venom is essential for antivenom production in Brazil; however, the impacts of hepatic neoplasms on venom composition remain unexplored. This study aimed to evaluate whether hepatic neoplasms and/or cysts influence the biochemical profile and enzymatic activity of venoms from five Bothrops species. Venoms from 17 snakes - 8 with hepatic alterations and 9 healthy - were analyzed using SDS-PAGE, Western blotting, ELISA, RP-HPLC, and enzymatic activity assays. The results revealed high intra- and interspecific variability, with no consistent pattern linking the presence of neoplasms to significant changes in venom protein composition or enzymatic activities. Occasional differences were observed in proteolytic, phospholipase A2, L-amino acid oxidase activities and Minimum Coagulant Dose in some individuals; however, these were not directly attributable to hepatic pathology. Immunoreactivity with the commercial antibothropic antivenom remained effective in all samples. The study concludes that hepatic neoplasms or cysts do not compromise venom biochemical integrity for antivenom production, reinforcing the viability of using these individuals for venom extraction in captivity for public health purposes. (AU) | |
| Processo FAPESP: | 21/11707-4 - Avaliação de métodos alternativos in vitro à utilização de animais para os ensaios in vivo no estudo de venenos de serpentes: princípio dos 3R's |
| Beneficiário: | Anita Mitico Tanaka-Azevedo |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 24/02642-4 - Perfil ontogenético do veneno e potencial de bioprospecção das toxinas three-finger da serpente Naja kaouthia |
| Beneficiário: | Anita Mitico Tanaka-Azevedo |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |