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Autor(es):
Nikolaou, Sofia ; Chaves, Otavio A. ; Bertoloni, Renan R. ; Iglesias, Bernardo A.
Número total de Autores: 4
Tipo de documento: Artigo Científico
Fonte: REVISTA VIRTUAL DE QUIMICA; v. 17, n. 4, p. 9-pg., 2025-07-01.
Resumo

The mu -Oxo-trinuclear ruthenium acetates are promising metallodrug candidates and have been evaluated for their cytotoxicity against certain cancer cell lines, as well as for their anti-parasitic activity against T. cruzi and vasodilator profile. Therefore, the interaction between fish sperm deoxyribonucleic acid (fs-DNA) and the clusters [Ru3O(CH3COO)6(pic)3]PF6 (1), [Ru3O(CH3COO)6(pic)2(H2O)]PF6 (2), [Ru3O(CH3COO)6(pic)2(CO)] (3), [Ru3O(CH3COO)6(py)2(H2O)]PF6 (4), and [Ru3O(CH3COO)6(H2O)3]PF6 (5) (pic 3-picoline; py pyridine) was evaluated by steady-state fluorescence and viscosity measurements and molecular docking calculations. The capacity of compounds 1-5 to displace the probes ethidium was moderate to weak, with Stern-Volmer quenching constant (Ksv) values in the order of 103 to 104 M-1. The highest constants consistently occurred for the DAPI probe, indicating that all compounds are minor groove binders, agreeing with the molecular docking trend, which also showed that van der Waals forces were mostly responsible for the interaction. Compounds 2, 4, and 5, containing a labile water molecule in their structure, moderately changed the fs-DNA viscosity, signaling the possibility of coordinative binding. Finally, variation in hydrophobicity (presence or absence of a methyl group in the structure of the ligands) and charge did not lead to significant differences in the interaction profile. (AU)

Processo FAPESP: 22/03478-8 - Reatividade de compostos polinucleares de rutênio com potencial aplicação biológica
Beneficiário:Sofia Nikolaou
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 22/08085-4 - Estudo da dinâmica do estado excitado e fotoclivagem de DNA de um complexo de coordenação fotossensibilizador com um ligante intercalador
Beneficiário:Renan Ribeiro Bertoloni
Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Mestrado
Processo FAPESP: 18/18060-3 - Uso de estruturas supramoleculares inorgânicas com vistas ao desenvolvimento de moléculas funcionais e liberação controlada de espécies bioativas
Beneficiário:Sofia Nikolaou
Modalidade de apoio: Auxílio à Pesquisa - Regular