Genotoxicity of corrosion eluates obtained from orthodontic brackets in vitro

Autor(es)

Angelieri, Fernanda ^[1] ; Marcondes, Joao Paulo C. ^[2] ; de Almeida, Danielle Cristina ^[2] ; Salvadori, Daisy M. F. ^[2] ; Ribeiroe, Daniel A. ^[3]

Afiliação do(s) autor(es)

^[1] Sao Paulo Methodist Univ, Dept Orthodont, Sao Paulo - Brazil

^[2] Sao Paulo State Univ, UNESP, Botucatu Med Sch, Dept Pathol, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, UNIFESP, Dept Biosci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: AMERICAN JOURNAL OF ORTHODONTICS AND DENTOFACIAL ORTHOPEDICS; v. 139, n. 4, p. 504-509, APR 2011.
• Citações Web of Science: 13

Resumo

Introduction: The purpose of this study was to evaluate whether corrosion eluates obtained from commercially available orthodontic brackets are able to induce genetic damage in vitro. Material and Methods: Genotoxicity was assessed by the single cell gel (comet) assay using Chinese hamster ovary (CHO) cells. The following orthodontic metallic brackets were used: Morelli (Sorocaba, Brazil); Abzil (Sao Jose do Rio Preto, Brazil); Dentaurum (Pforzheim, Germany); and 3M Unitek (Puchheim, Germany). Each dental bracket was submitted to a corrosion process in a solution containing equal amounts of acetic acid and sodium chloride at 0.1 M concentration for 1, 3, 7, 14, 21, 35, and 70 days. CHO cells were exposed to eluates for 30 minutes at 37 degrees C. The negative control was treated with the same solution used for corrosion process for 30 minutes at 37 degrees C. Independent positive control was performed with methyl methanesulfonate (MMS) (Sigma Aldrich, St. Louis, Mo) at 1 ug/mL for 1 hour. Results: None of the eluates was found to exhibit genotoxicity, regardless of the different commercial brands of orthodontic appliance used. Conclusions: In summary, our results indicate corrosion eluates obtained from orthodontic brackets do not induce genetic damage as assessed by single cell gel (comet) assay. (Am J Orthod Dentofacial Orthop 2011; 139: 504-9) (AU)

• Processo FAPESP: 07/00345-7 - Danos no DNA promovidos por íons metálicos provenientes de aparelhos ortodônticos fixos: análises in vitro e in vivo
• Beneficiário: Daniel Araki Ribeiro
• Modalidade de apoio: Auxílio à Pesquisa - Regular