| Texto completo | |
| Autor(es): |
Dos Santos, Maria Ines
;
Dombrowski, Jamille Gregorio
;
Hoffmann-Veltung, Henriette
;
Quintana, Maria Del Pilar
;
Marinho, Claudio Romero Farias
;
Hviid, Lars
;
Lopez-Perez, Mary
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Infection and Immunity; v. N/A, p. 13-pg., 2025-10-31. |
| Resumo | |
Placental malaria (PM) causes mortality and severe morbidity in areas with stable Plasmodium falciparum transmission. The selective placental accumulation of P. falciparum-infected erythrocytes (IEs) is mediated by VAR2CSA, a PfEMP1-type parasite ligand that binds exclusively to placenta-restricted CSA. VAR2CSA-specific IgG is therefore generally restricted to women exposed to P. falciparum infection during pregnancy. However, widespread acquisition of VAR2CSA-reactive IgG outside pregnancy among Colombian and Brazilian individuals has been reported, supposedly due to cross-reactivity between VAR2CSA and the P. vivax-specific antigen PvDBP. Here, we measured levels and Fc-afucosylation of VAR2CSA-reactive IgG in plasma from pregnant Brazilian women at delivery, using full-length VAR2CSA (FV2) expressed in baculovirus-transfected insect cells (FV2BIC) or Chinese hamster ovary cells (FV2CHO) as well as the corresponding native antigen (IT4VAR04) on the IE surface. We also measured levels of IgG specific for GLURP (P. falciparum-specific) and PvDBP (P. vivax-specific). FV2CHO-specific IgG levels were lower than FV2BIC-reactive IgG levels. Furthermore, only FV2CHO-specific IgG was restricted to women exposed to P. falciparum during pregnancy. Levels of PvDBP-specific IgG were significantly higher among P. vivax-exposed pregnant women but did not correlate with FV2-specific IgG levels. Finally, FV2CHO-specific IgG was markedly Fc-afucosylated in contrast to FV2BIC-reactive IgG. Our findings caution against using levels of IgG reacting with recombinant proteins expressed in insect cells as a measure of exposure to VAR2CSA during pregnancy, at least in South America. Furthermore, our data do not support the hypothesis that exposure to PvDBP induces IgG that cross-reacts with VAR2CSA and contributes to protection against PM. (AU) | |
| Processo FAPESP: | 24/10186-9 - I-GAME: Genômica integrada e IA como ferramenta para eliminação da malária no Brasil |
| Beneficiário: | Cláudio Romero Farias Marinho |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 21/07170-5 - Identificação de biomarcadores para restrição do crescimento fetal durante a Malária gestacional |
| Beneficiário: | Maria Ines dos Santos |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 24/05344-4 - Associação da reatividade de IgG para VAR2CSA PfEMP1 de comprimento total com desfechos gestacionais numa região endêmica de malária |
| Beneficiário: | Maria Ines dos Santos |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado Direto |
| Processo FAPESP: | 19/12068-5 - Identificação de biomarcadores preditivos de disfunção placentária na Malária |
| Beneficiário: | Jamille Gregório Dombrowski |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |